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Record W4404241566 · doi:10.1101/2024.11.07.622524

A global view of the RNA-binding and regulatory protein landscape in <i>Caenorhabditis elegans</i>

2024· preprint· en· W4404241566 on OpenAlexafffund
John Laver, John A. Calarco

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2024
Typepreprint
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicRNA Research and Splicing
Canadian institutionsUniversity of Toronto
FundersNatural Sciences and Engineering Research Council of CanadaCanadian Institutes of Health ResearchUniversity of Toronto
KeywordsCaenorhabditis elegansRNA-binding proteinBiologyRNAComputational biologyCell biologyGeneticsGene

Abstract

fetched live from OpenAlex

Abstract Post-transcriptional regulation of gene expression is essential for the correct development and functioning of an organism. This regulation is coordinated by a collection of proteins that work together to determine an RNA’s post-transcriptional fate. Here, we provide a global overview of the RNA regulatory protein landscape in Caenorhabditis elegans , to provide insight into the coordination of post-transcriptional regulatory activities in the context of a multicellular organism. First, we have curated a comprehensive list of all known and putative RNA regulatory proteins encoded in the C. elegans genome, classified based on domain and functional annotations and published experimental data. Second, using protein-protein interaction data in the STRING database, we created a putative RNA regulatory protein interaction network that highlighted known RNA regulatory complexes, and leveraged this network to identify an additional 138 known and putative RNA regulators previously unannotated in C. elegans . Finally, we examined the tissue- and developmental-stage-specific expression of RNA regulators using published transcript expression data, which revealed strong expression in the gonad for a majority, as well as dozens expressed specifically in each of the major somatic C. elegans tissues. Taken together, this work will provide a valuable resource for future studies of RNA biology in C. elegans .

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.005

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.006
GPT teacher head0.220
Teacher spread0.214 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2024
Admission routes2
Has abstractyes

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