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Record W4404324825 · doi:10.1080/23802359.2024.2427109

Phylogenetic analysis of the complete mitochondrial genome of the orange-winged sulphur butterfly <i>Dercas nina</i> Mell 1913 (Insecta: Lepidoptera: Pieridae: Coliadinae)

2024· article· en· W4404324825 on OpenAlexafffund
Arlene M. Agcaoili, Nabiha Ameena, Dexter Andres, Rhey Caners, Nicole J. Croitor, Kaesy Lynne Enns, Oleksandra Fedorova, Hannah A. Garber, Sarah D. Gregoire, Tenley E. Ilnisky, Annie Jiang, Feryal Ladha, Alexandria Martin, Mary A. McAuley, Cassidy McNeill, Senudi D. Nanayakkara, N. Nguyen, DA Peters, Madison N. Poitras, Dhruvi V. Prajapati, Camille Préfontaine, Ravindu V. Rajapaksha, Pratyaksh Singhal, Cedey Souriyavong, Kayla R. Talabis, Yi Tan, J. Tang, Kailey W. Tkach, Ashley Tohms, Cameron G. Tramley, Josh Treftlin, Diya Ukani, Ethan A. Vallelly, Patrick V. Wiens, C. A. T. Yee, Yu Ke, Jeffrey M. Marcus

Bibliographic record

VenueMitochondrial DNA Part B · 2024
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicLepidoptera: Biology and Taxonomy
Canadian institutionsUniversity of Manitoba
FundersNatural Sciences and Engineering Research Council of Canada
KeywordsPieridaeLepidoptera genitaliaButterflyBiologyPhylogenetic treeMitochondrial DNAZoologyEcologyGeneticsGene

Abstract

fetched live from OpenAlex

Dercas nina Mell Citation1913 (Pieridae) is a little-studied butterfly species endemic to China that flies primarily in the forest canopy. Genome skimming by Illumina sequencing allowed assembly of 146,702 reads for complete 1471.3-fold mean coverage of the circular 15,264 bp mitogenome from D. nina consisting of 82.1% AT nucleotides. A gene order typical of butterflies was recovered consisting of 13 protein-coding genes, 22 tRNAs, two rRNAs, and a predicted control region. The Dercas nina COX1 open reading frame begins with atypical start codon CGA. Six protein-coding genes (COX1, COX2, ND2, ND3, ND4, ND5) with single-nucleotide (T) stop codons, and two protein-coding genes (ATP6, ATP8) with two-nucleotide (TA) stop codons encoded in the DNA were inferred to be completed by adenine nucleotides from the Poly-A tail of the mRNA. Bayesian’s phylogenetic reconstruction places the D. nina and D. lycorias mitogenomes as sister clades. Dercas mitogenomes were sister to those from genus Colias in the monophyletic subfamily Coliadinae. The mitogenome phylogeny is consistent with previous molecular phylogenetic hypotheses based on other markers, but differs somewhat from a morphology-based hypothesis that suggested that Dercas was more closely related to genus Gonepteryx. This may falsify the hypothesis or may instead reflect mitochondrial-nuclear phylogenetic discordance.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.004
Threshold uncertainty score0.009

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.001
Bibliometrics0.0020.002
Science and technology studies0.0010.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.016
GPT teacher head0.228
Teacher spread0.212 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations2
Published2024
Admission routes2
Has abstractyes

Explore more

Same venueMitochondrial DNA Part BSame topicLepidoptera: Biology and TaxonomyFrench-language works237,207