Yeast cell wall extract as a strategy to mitigate the effects of aflatoxin B1 and deoxynivalenol on liver and intestinal health, and gut microbiota of largemouth bass (Micropterus salmoides)
Bibliographic record
Abstract
The increasing use of plant-based raw materials in aquatic feed has led to a growing concern about the harmful effects of mycotoxins on aquatic animals. This study aimed to evaluate the supplementation of yeast cell wall extract (YCWE) on the growth performance, liver and intestine health, and gut microbiota of largemouth bass ( Micropterus salmoides ) fed with aflatoxin B1 (AFB1), and deoxynivalenol (DON) contaminated feeds. AFB1, DON and YCWE were supplemented to the basic formula feed at different concentrations to develop eight dietary groups and M. salmoides were fed for 8 weeks. Results showed that the inclusion of YCWE in mycotoxins-contaminated diets had no effect on the growth performance but significantly affected the immunity and antioxidant capacity of M. salmoides ( P < 0.05). The overall changes in serum biochemical indicators were not significant, except that the ALT/AST activities were significantly higher ( P < 0.05) under the mycotoxin challenge, and significantly lower in 0.2 % YCWE treatment group. Furthermore, the expression of genes related to liver and intestine inflammation (pro-inflammatory factors nf-kb, tnf-a, il-1β, il-6, il-8, ifn-γ and anti-inflammatory factors il-10, tgf-β ), and antioxidant regulatory factors nrf2/keap1 showed an up-regulation trend, and the content of the lipid metabolite MDA was notably higher under mycotoxin challenge and YCWE treatments ( P < 0.05). Moreover, the supplementation of YCWE significantly alleviated the inflammatory responses ( P < 0.05), enhanced the activities of antioxidant enzymes (SOD, CAT) ( P < 0.05) and the expression of tight junction proteins genes occludin , claudin4 , and zo- 1, and reduced the content of MDA, suggesting that mycotoxin challenge tested herein could activate the inflammatory response and lead to oxidative stress while YCWE could improve liver and intestinal health in mycotoxin challenged fish. Additionally, metataxonomic profiling of the gut microbiota showed that YCWE supplemented fish had higher bacterial richness and diversity, suggesting its role in reducing gut microbial dysbiosis . In summary, mycotoxins had negative effects on the health of M. salmoides , while the supplementation of YCWE could alleviate such negative effects in a dose-dependent manner, demonstrating that YCWE can be a remediation strategy for mycotoxins decontamination in aquaculture.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".