Cross-amplification of microsatellite markers on the endangered tree species <i>Castanopsis argentea</i> from other <i>Castanopsis</i> species: a valuable tool for genetic diversity analysis
Bibliographic record
Abstract
Castanopsis argentea is an endangered tree naturally distributed in Java and Sumatera Island and considered a keystone species in the submontane forest ecosystem. A laboratory study was conducted to test the cross-amplification potential of seven pairs of Castanopsis cuspidata var. sieboldii microsatellite markers to be used for Castanopsis argentea. The rate of cross-amplification success, average number of alleles per locus (NA), observed heterozygosity (HO), expected heterozygosity (HE), and fixation index (FIS) were determined, and cross-amplification potential was investigated. The rate of amplification success was 85.71% (6 out of 7 markers were successful amplified), NA was 7.67, HO ranged from 0.467 to 0.923, HE ranged from 0.441 to 0.745, and the fixation index (FIS) ranged from −0.413 to 0.278. Results indicate the high transferability of C. cuspidata var. sieboldii microsatellite markers to C. argentea. Six markers were suitable for genetic diversity and population genetic studies on C. argentea.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".