Cryo-protective effect of ice-binding proteins produced by Pseudomonas fluorescens AQP671 on wholegrain wheat bread dough during freezing and frozen storage
Bibliographic record
Abstract
Ice-binding proteins (IBPs) of cold-induced Pseudomonas fluorescens AQP671 were assessed for their potential as cryoprotective agents in frozen wholegrain wheat dough during freezing and 8 weeks of frozen storage. The protein solution exhibited significant ice-recrystallization inhibition activity at total protein concentrations of 0.1 g/L and above. The addition of IBPs improved the yeast survival rates in the dough post-freezing on average 7.8% compared to the untreated samples. Additionally, yeast survival rates were stable during 8 weeks of frozen storage in samples that had at least 5% (0.09 g/kg) of the total dough weight supplemented with the protein solution. As a result, the rising capacity of the dough was improved by the addition of ice-binding proteins, correlating to their concentration in the dough. Additionally, the freeze tolerance of the starch granules was maintained for at least 6 weeks for all of the IBP-treated samples. Furthermore, the ice-binding proteins had a positive effect on the strength of the dough after thawing, indicating less damage to the gluten structure and improved the overall texture and sensory parameters of the final bread product. • P. fluorescens AQP671 IBPs have high ice recrystallization inhibition activity. • IBPs can protect the yeast cells during freeze-thaw cycles. • IBPs decrease the degradation of starch granules during frozen storage. • The supplementation of IBPs may help maintain the texture and sensory attributes of bread made from frozen dough.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".