Gene Silencing via Ingestion of Double-Stranded RNA in Wireworm of Agriotes Species
Bibliographic record
Abstract
Wireworms are the most destructive soil insect pests affecting horticultural crops. The damage often renders them unsuitable for commercial purposes, resulting in substantial economic losses. RNA interference (RNAi) has been broadly used to inhibit gene functions to control insect populations. It employs double-stranded RNA (dsRNA) to knockdown essential genes in target organisms, rendering them incapable of development or survival. Although it is a robust approach, the primary challenges are identifying effective target genes and delivering their dsRNA into wireworms. Thus, the present study established a liquid ingestion methodology that efficiently delivers dsRNA into wireworms. We then investigated the effects of four target genes on wireworm mortality. The highest mortality rate reached 50% when the gene encoding vacuolar ATPase subunit A was targeted. Its transcript content in the fed wireworms was also significantly reduced. The mortality rates of the other three target genes of vacuolar ATPase subunit E, beta-actin, and chitin synthase 1 were 28%, 33%, and 35%, respectively. This is the first report demonstrating an efficient feeding methodology and the silencing of target genes in wireworms. Our findings indicate that RNAi is an effective alternative method for controlling the wireworm pest, and can be used to develop field treatment strategies.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".