Transcriptome analysis and physiological response to heat and cold stress in flax (Linum usitatissimum L) at the seedling stage
Bibliographic record
Abstract
Exposure to low temperatures renders flax seedlings vulnerable to oxidative damage, leading to delayed flowering, while high temperatures hinder seedling growth and negatively impact pollen viability. The seedling stage is particularly sensitive to environmental stressors, which can result in decreased flax yield and compromised seed oil quality. In this study, phenotypic assessments, along with physiological, biochemical, and transcriptomic analyses, were conducted on flax plants subjected to both high and low temperature stress, followed by 6 d recovery period. Results showed that the length and weight of seedlings and shoots were greatest in flax plants that had recovered from low temperature stress. After exposure to low temperature stress, flax seedlings exhibited the highest relative water content (RWC) and relative water loss (RWL) measuring 66.56% and 93.34%, respectively. The levels of Pro, SOD, CAT, POD, and nine phytohormones were significantly elevated compared to the control, whereas MDA levels were notably declined. A total of 43,471 genes were identified in the transcriptome data, Among these 32,319 exhibited sighificant differential expression. GO analysis highlighted enrichment in biological processes, cellular components, and molecular functions. KEGG analysis showed enrichment in plant hormone signal transduction and secondary metabolite biosynthesis. Moreover, differentially expressed genes associated with phytohormone synthesis and signal transduction were analyzed. The expression level of salicylic acid (SA) genes was significantly upregulated under high temperature stress, whereas jasmonic acid (JA) genes were significantly up-regulated under low temperature stress. These findings will elucidate the intricate regulatory mechanisms of osmoregulatory factors, antioxidant enzymes, and endogenous plant hormones in flax's response to high and low temperature stress, offering valuable insights for the selection and breeding of resistant flax varieties and the enhancement of genetic resources. • Flax ( Linum usitatissimum L.) is one of the most important oil and economic crops in China. • Temperature is crucial throughout the growth and development of plants. • Genes related to jasmonic acid (JA) exhibited significant up-regulation under low-temperature stress. • Genes related to salicylic acid (SA) exhibited significant up-regulation under high-temperature stress. • This finding deepen our understanding of flax seedlings response to temperature stress.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".