Systematic analysis of WOX transcription factor family in the biodiesel tree Lindera glauca and functional characterization of LgWOX24 in root development
Bibliographic record
Abstract
The WUSCHEL-related homeobox (WOX) gene family is vital for plant growth and development. Despite its importance, more research is needed on the WOXs in economically significant species like Lindera glauca , a tree with oil-rich fruits and potential as a biodiesel feedstock. Understanding the WOX family in L. glauca could enhance its economic value, yet a comprehensive analysis is needed, leaving a gap in our knowledge of these genes' roles. Here, we performed a genome-wide analysis to identify and characterize the WOX family in L. glauca . We identified 30 LgWOXs , categorized into three clades. Sequence alignment confirmed that these LgWOX proteins have a conserved structure, suggesting similar functions. Synteny and chromosomal distribution analyses indicated that both whole-gene and segmental duplications contributed to the expansion of the LgWOX family. Expression pattern analysis revealed that LgWOX3 and LgWOX24 are predominantly expressed in L. glauca roots, indicating a role in root development. Transient overexpression of LgWOX24 resulted in reduced expression of genes related to hormone polar transport and increased levels of abscisic acid regulators, suggesting that LgWOX24 negatively regulates root growth. These findings provide insights into the roles of WOXs in L. glauca , particularly in root development. By elucidating the function of LgWOX24 , this study lays the groundwork for future bioengineering efforts to optimize root growth, potentially enhancing L. glauca 's utility as a biodiesel feedstock and its broader agricultural applications. • Thirty WOXs were identified in the Lindera glauca genome. • Transcription levels of LgWOXs varied significantly across different tissues. • Whole-gene and segmental duplications contributed to the expansion of LgWOXs family. • LgWOXs expressed specifically in roots are localized in the nucleus. • LgWOX24 negatively regulates root growth in L. glauca .
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".