Towards the standardization of Hydra vulgaris bioassay for toxicity assessments of liquid samples
Bibliographic record
Abstract
The Hydra vulgaris bioassay is recognized as sensitive invertebrate test species for toxicity assessment of real-life environmental mixtures for enforcement and monitoring investigations. The purpose of this study was to characterize the intra-laboratory variability, study the influence of environmental variables (temperature, luminosity, inter-individual and day of analysis) on ZnSO 4 toxicity, a reference model toxicant for hydra. The sublethal (effect concentration for 50 % of hydra-EC50) and lethal (lethal concentration for 50 % of hydra-LC50) were determined based on characteristic morphological changes for this species. The influence of water hardness, ammonia and dissolved oxygen for over 50 real-life environmental liquid mixtures (effluents and leachates) were examined and compared with rainbow trout and Daphnia magna acute lethality tests. A control chart for Zn was developed from over 40 trials yielding an 96 h LC50 of 0.7 mg/L (0.66–0.77 95 % confidence interval-CI) and EC50 of 0.19 mg/L (0.17–0.21 95 % CI). The influence of 8 different analysts, the trial days and luminosity did not significantly influence the LC50 and EC50. Only higher temperature significantly decreased the toxicity of ZnSO 4 within 15–30 °C range. The hydra bioassay was then practiced on more than 50 real-life effluents/leachates and compared with the 96 h rainbow trout and 48 h Daphnia magna survival tests. The data revealed that water hardness, dissolved oxygen and ammonia were not significantly correlated with either the LC50 or EC50 values. Moreover, the hydra LC50 data predictive (rank correlation of 0.6) the rainbow trout LC50 with the absence of false negatives. The hydra data were at least as if not more sensitive than the rainbow trout LC50 making it a relevant alternative method to reduce fish use for screening potentially toxic environmental mixtures. • The Hydra vulgaris assay was standardized using ZnSO4. • The hydra sublethal and lethal toxicity of miscellaneous mixtures were examined. • The hydra was more sensitive than rainbow trout and Daphnia magna. • The hydra toxicity data compared favorably with the rainbow trout juvenile toxicity.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.006 | 0.004 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.001 | 0.002 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".