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Record W4405984779 · doi:10.1021/acsabm.4c01073

Coating of Threads with Fluorescent Curli Fibers for pH Sensing

2025· article· en· W4405984779 on OpenAlex
Dalia Jane Saldanha, Noémie‐Manuelle Dorval Courchesne

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueACS Applied Bio Materials · 2025
Typearticle
Languageen
FieldEngineering
TopicBiosensors and Analytical Detection
Canadian institutionsMcGill University
FundersFonds de recherche du Québec – Nature et technologiesNatural Sciences and Engineering Research Council of CanadaJohnson and JohnsonCanada Foundation for InnovationCentre québécois sur les matériaux fonctionnelsCanada Research ChairsFaculty of Engineering, McGill UniversityMcGill University
KeywordsCoatingThread (computing)Materials scienceNanotechnologyFluorescenceBiosensorComputer scienceOptics

Abstract

fetched live from OpenAlex

Threads coated with bioresponsive materials hold promise for innovative wearable diagnostics. However, most thread coatings reported so far cannot be easily customized for different analytes and frequently incorporate non-biodegradable components. Most optically active thread coatings rely on dyes, which often exhibit irreversible responses. In this work, we propose a biosensing coating for threads using curli fibers. Curli fibers are self-assembling fibers of the protein CsgA that can be genetically engineered to sense rapidly evolving diagnostic targets. We first established a simple electrostatic-mediated absorption protocol for coating anionic cotton threads with anionic curli fibers using an intervening cationic chitosan layer. We applied this protocol to two types of pH-sensing curli fibers, displaying either fluorescent pHuji or mCitrine proteins. This process ensures extensive curli coating over the entire thread surface using only water-based solvents. The resulting protein-coated threads are moderately hydrophobic, stretchable, and can monitor pH changes in real time through fluorescence. The coatings are also stable and functional on the surface for over 25 cycles of use, highlighting their potential for reusable practical applications. This straightforward and adaptable protocol can be extended to coat threads with diverse sensing and responsive capabilities for intelligent clothing.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.007
Threshold uncertainty score0.358

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.007
GPT teacher head0.207
Teacher spread0.200 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it