Nonfibrillar Dutch mutant amyloid beta (Aß) aggregates (oligomers) are associated with aging‐related synaptic dysfunction
Bibliographic record
Abstract
Abstract Background Clinicopathological studies of Alzheimer’s disease (AD) have demonstrated that synaptic or neuronal loss and clinical cognitive decline do not reliably correlate with fibrillar amyloid burden. We created a transgenic mouse model overexpressing Dutch (E693Q) mutant human amyloid precursor protein (APP) driven by the pan‐neuronal Thy1 promoter. Accumulation of APP carboxyl‐terminal fragments was observed in the brains of these mice, which develop an impaired learning phenotype directly proportional to brain oAβ levels. Method Male and female TgAPPE693Q mice and wildtype controls were compared using learning behavioral studies, immunocytochemistry, transmission electron microscopy, electrophysiology, protofibril‐specific assays, and single cell RNA sequencing. Result Brain levels of nonfibrillar oAβ in Dutch mice were shown to increase aging‐dependently using A11 immunocytochemistry and FITC‐cyclic peptide (FITC‐CP‐2) microscopy. Two assays excluded the presence of protofibrils. Electrophysiological characterization of hippocampal synapses in Dutch and wildtype mice at ∼7 and ∼11 months revealed no change in basal excitatory transmission, consistent with normal density and morphology of mGluR2/3+ synapses in hippocampal CA1 of the same mice. One exception was increased postsynaptic density in non‐perforated mGluR‐2/3+ synapses in the Dutch mice. Functional characterization of the presynaptic terminal showed abnormalities in post‐tetanic potentiation, synaptic fatigue, and synaptic replenishment after depletion in Dutch mice. Single cell RNA‐seq to elucidate cell‐type specific transcriptional responses to oAβ revealed altered transcriptional profiles in multiple cell types. Unexpectedly, no obvious differences existed between profiles of microglia from Dutch compared to those from wildtype mice. Excitatory neurons showed the most altered profile which was associated with ‘protein translation’ and ‘oxidative phosphorylation’. Ultrastructural analysis of presynaptic mitochondria at excitatory synapses revealed fewer mitochondria in the presynaptic terminals of Dutch mice. Conclusion The profound learning behavior deficits in Dutch mice are associated with presynaptic functional deficits and mitochondrial abnormalities in excitatory neurons of the hippocampus. Nonfibrillar oAβ deposits were revealed by co‐localization of A11 immunoreactivity with FITC‐CP‐2 microscopy. Mice accumulating only oAβ may be especially useful for further characterization of the oligomer‐specific cyclic azaglycine PET tracer Lys (64Cu/NOTA)1]‐CP‐7 that shows robust PET signal from 44‐day‐old presymptomatic 5xFAD mice [Habashi, M. et al. Proc. Natl. Acad. Sci. U.S.A. 2022].
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".