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Record W4406057600 · doi:10.1101/2025.01.03.631187

Regulation of the lncRNA <i>malat1</i> /Egr1 Axis by Wnt, Notch and TGF-β signaling: A Key Mechanism in Retina Regeneration

2025· preprint· en· W4406057600 on OpenAlexaff
Sharanya Premraj, Poonam Sharma, Pooja Shukla, Kshitiz Yadav, Omkar Desai, Rajesh Ramachandran

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2025
Typepreprint
Languageen
FieldMedicine
TopicCorneal Surgery and Treatments
Canadian institutionsInstitute of Infection and Immunity
Fundersnot available
KeywordsMALAT1Mechanism (biology)Wnt signaling pathwayNotch signaling pathwayKey (lock)Cell biologyRegeneration (biology)Signal transductionBiologyLong non-coding RNAGeneticsRNAGenePhysics

Abstract

fetched live from OpenAlex

Abstract Adult zebrafish retinas rely on the resident Müller glia to maintain homeostasis and enable regeneration. Retina regeneration remains incomplete in mammals despite extensive efforts to emulate zebrafish regenerative conditions. Many studies have examined the reprogramming of zebrafish Müller glia cells, which is necessary for regeneration driven by regeneration-associated gene expression. Here, we show that the lncRNA malat1 , crucial for many biological functions, plays essential roles during retina regeneration. We demonstrate that malat1 functions through an Egr-dependent axis, modulated by Wnt, Notch, and TGF-β signaling pathways, and is necessary for effective retina regeneration. Moreover, we uncover that the antisense lncRNA talam1 , which regulates malat1 availability, is differentially regulated in zebrafish and mice, highlighting species-specific gene regulatory mechanisms after retinal injury. Cells with active TGF-β signaling stabilize Malat1 in mice while the same signaling destabilizes malat1 in zebrafish. Taken together, our work uncovers a new role for the malat1 /Egr1 axis in necessitating retina regeneration, which may have important implications for differential regenerative ability in vertebrates.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.010

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0030.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.214
Teacher spread0.203 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

Explore more

Same venuebioRxiv (Cold Spring Harbor Laboratory)Same topicCorneal Surgery and TreatmentsFrench-language works237,207