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Compositional and functional diversity of minimal primitive coacervates in a nucleic acid-peptide world

2025· preprint· en· W4406424764 on OpenAlexafffund
Karina K. Nakashima, Fatma Zohra Mihoubi, Jagandeep S. Saraya, Kieran Russell, Fidan Rahmatova, James D. Robinson, Maria Julia Maristany, Jan Huertas, Roger Rubio‐Sánchez, Rosana Collepardo‐Guevara, Derek K. O’Flaherty, Claudia Bonfio

Bibliographic record

VenueChemRxiv · 2025
Typepreprint
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMuscle metabolism and nutrition
Canadian institutionsUniversity of Guelph
FundersBiotechnology and Biological Sciences Research CouncilDirectorate for Biological SciencesChina Scholarship CouncilGovernment of the United KingdomUniversité de StrasbourgAgence Nationale de la RechercheHORIZON EUROPE Framework ProgrammeNederlandse Organisatie voor Wetenschappelijk OnderzoekNatural Sciences and Engineering Research Council of CanadaInternational Business Machines CorporationEuropean CommissionEngineering and Physical Sciences Research CouncilUK Research and Innovation
KeywordsNucleic acidCoacervateDiversity (politics)Functional diversityPeptide nucleic acidPeptideChemistryComputational biologyCombinatorial chemistryBiochemistryBiologyEcologyPolitical science

Abstract

fetched live from OpenAlex

The RNA-peptide world hypothesis postulates the early co-evolution of RNA and peptides, leading to the emergence of non-enzymatic RNA replication and peptide synthesis. Although nucleotides and amino acids were shown to form and polymerise under prebiotic conditions, the origin of their synergy, ubiquitously preserved in the central dogma of modern biology, remains unclear. We propose that the cooperation between DNA, RNA and peptides might have stemmed from their co-localisation in early compartments. Here we show that heterogeneous mixtures of prebiotic oligonucleotides and peptides spontaneously assemble into primitive coacervates. Experimental and computational studies reveal that peptide/nucleic acid coacervates are more robust and form over a broader range of conditions than peptide/peptide analogues. Notably, RNA-based coacervates exhibit exceptional stability and, in the presence of DNA, minimal viscosity, facilitating the diffusion of reactive oligonucleotides and supporting prebiotic RNA chemistry. Our findings suggest that coacervation may have occurred in the early on the evolutionary timeline, fostering the emergence of a nucleic acid-peptide world. This study provides new insights into the prebiotic role of coacervates, reconsidering their significance in the origins of life and the emergence of primitive replication and translation systems.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.435
Threshold uncertainty score0.751

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.001
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.018
GPT teacher head0.236
Teacher spread0.218 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations5
Published2025
Admission routes2
Has abstractyes

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