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Record W4406512436 · doi:10.1101/2025.01.16.25320681

Assessing the performance and suitability of wastewater based-surveillance for SARS-CoV-2 RNA in public schools

2025· preprint· en· W4406512436 on OpenAlexaff
Nicole Acosta, Alexander Buchner Beaudet, Paul Westlund, Jia Hu, Navid Sedaghat, Puja Pradhan, Lawrence Man, Jordan Hollman, María A. Bautista, Barbara J. Waddell, Janine McCalder, Matthew Penney, Jianwei Chen, Jon Meddings, Gopal Achari, M. Cathryn Ryan, Elizabeth A. Wood, Jason Cabaj, Rhonda G. Clark, Kevin J. Frankowski, Casey R. J. Hubert, Michael D. Parkins

Bibliographic record

VenuemedRxiv · 2025
Typepreprint
Languageen
FieldMedicine
TopicSARS-CoV-2 detection and testing
Canadian institutionsUniversity of Calgary
Fundersnot available
KeywordsWastewaterCoronavirus disease 2019 (COVID-19)Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Environmental scienceBusinessComputer scienceEnvironmental engineeringMedicineInternal medicine

Abstract

fetched live from OpenAlex

Abstract Municipal wastewater-based surveillance (WBS) programs for SARS-CoV-2 were valuable tools for epidemiological modelling and informing COVID-19 health policy during the pandemic. We conducted a “near to source” study to assess the capacity and performance of SARS-CoV-2 WBS programs in schools relative to municipal wastewater treatment plants (WWTP). Only 4/17 schools screened had plumbing systems that were amenable to WBS. From December 2020 - March 2021 composite wastewater collected 2X/week from four schools and three municipal WWTP were assessed for SARS-CoV-2 and fecal biomarkers. Schools had lower rates of successful sample collection relative to WWTP (44.7% vs 100%, p<0.001). In a time of low COVID-19 activity, 13/64 of school samples were positive of SARS-CoV-2-N1 vs 100% of WWTP. SARS-CoV-2-N1 detected in school wastewater associated with and preceded clinically identified infections, but did not correlate with rates of overall absenteeism. Notably levels of SARS-CoV-2-N1 and fecal biomarkers were markedly lower in wastewater from schools relative to WWTP. While our SARS-CoV-2 WBS program in schools did generate a leading signal relative to clinical disease, the significantly lower levels of SARS-CoV-2 and fecal biomarkers suggests that toileting habits of children who may avoid defecation at school adversely impact school-based WBS of targets shed in feces.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.002
metaresearch head score (Gemma)0.002
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.549
Threshold uncertainty score0.757

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0020.002
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.080
GPT teacher head0.350
Teacher spread0.270 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2025
Admission routes1
Has abstractyes

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