HSP10 and HSP20 involved in the adaptation of Eriocheir sinensis under air exposure stress
Bibliographic record
Abstract
Air exposure stress is one of the most common challenges faced by aquatic animals. Eriocheir sinensis , as a migratory crustacean that inhabits a variety of environments, is an excellent model for studying air exposure adaptation in aquatic animals. Small heat shock proteins (sHSPs), HSP10 and HSP20, as highly conserved molecular chaperones, are often involved in various stresses. Therefore, we aim to explore the functional information of sHSPs in stress resistance and evolution in the E. sinensis . In this study, through the E. sinensis genome, five HSP10 and fourteen HSP20 sequences were identified. HSP20 sequences showed differently duplicated models including tandem duplications and proximal duplications. Four HSP10 and one HSP20 were not expressed in all transcriptome data and were identified as pseudogenes . It was thought that the production of these pseudogenes was caused by the absence of key motifs. Except for these pseudogenes, all genes were responsive to air exposure stress in hepatopancreas, indicating that HSP10 and HSP20 were involved in the response of E. sinensis to air exposure stress. RNA interference assay revealed that HSP20 response to air exposure stress were regulated by hypoxia inducible factor-1α (HIF-1α). The count difference of exon and intron in HSP20 could influence whether HSP20 was expressed in the gills or not under stress. Tandem duplications located on different chromosomes had differences in gene expression, which may result from selective pressure. These results should deepen our understanding of the stress resistance functions of sHSPs and offer new insights into the environmental adaptation of E. sinensis.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".