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Record W4406884599 · doi:10.1101/2025.01.27.635011

Passive symplastic phloem loading in the duckweed <i>Spirodela polyrhiza</i>

2025· preprint· en· W4406884599 on OpenAlexaff
Jiazhou Li, Johannes Liesche

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2025
Typepreprint
Languageen
FieldEnvironmental Science
TopicCoastal wetland ecosystem dynamics
Canadian institutionsInstitute of Aging
Fundersnot available
KeywordsPhloemBotanyBiology

Abstract

fetched live from OpenAlex

Abstract The lemnoidae, commonly called duckweeds, are a group of small, rapidly growing aquatic plants that play an important role in pond ecosystems and are used in biotechnological and remediation applications. While small, duckweeds feature phloem tissue in fronds and roots. To gain insight on duckweed phloem function, we investigated how sugar is loaded into the phloem sieve elements in the giant duckweed Spirodela polyrhiza . Genomes of S. polyrhiza and three other duckweeds do not feature genes for the sucrose transporters typically associated with active apoplastic phloem loading. Neither did a sucrose transporter inhibitor affect sucrose concentration in phloem exudate. Active symplastic phloem loading was excluded based on the conventional plasmodesmata configuration and absence of oligosaccharides in S. polyrhiza phloem. Instead, uniform plasmodesmata density along the phloem loading pathway indicated a passive symplastic phloem loading type. When plasmodesmata permeability was artificially reduced by hormone treatment, the sucrose concentration in the phloem exudate was reduced, highlighting the potential role of plasmodesmata regulation in setting carbon export rates in species with passive phloem loading. Our results identify S. polyrhiza as the first monocot species with passive phloem loading. Moreover, they indicate opportunities for optimization of duckweed growth.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.005

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.007
GPT teacher head0.193
Teacher spread0.186 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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