Influence of Vibrio anguillarum culture conditions on the efficacy of bacterin-based vaccines in lumpfish (Cyclopterus lumpus)
Bibliographic record
Abstract
Lumpfish ( Cyclopterus lumpus ) is used as cleaner fish to control sea lice infestations in Atlantic salmon ( Salmo salar ) farms across the North Atlantic. Vibrio anguillarum , the causative agent of vibriosis, is a recurrent bacterial pathogen affecting lumpfish. Bacterin-based vaccines are frequently used to control vibriosis in finfish, but their efficacy is not always consistent. Culture conditions significantly influence synthesis of bacterial outer membrane and secreted proteins, which are critical antigens, and thus impact the immunogenicity of bacterin-based vaccines. In this study, we assessed the effect of V. anguillarum culture conditions on vaccine efficacy in lumpfish. V. anguillarum was cultured under iron-limited at 15 °C, and iron-rich or iron-limited conditions at 28 °C with 2 % NaCl, and these cultures were used to prepare bacterins. A commercial vaccine was used as positive control, while PBS and PBS adjuvant were negative controls. Lumpfish were intraperitoneally immunized and challenged 12 weeks post-immunization with 10–100 times the LD 50 dose of V. anguillarum . Bacterins prepared from V. anguillarum grown under iron-limited conditions at 28 °C with 2 % NaCl and mixed with adjuvant conferred the highest protection compared to other preparations and commercial vaccines. In contrast, bacterins derived from V. anguillarum cultured under iron-limited conditions at 15 °C conferred the lowest protection. Reverse vaccinology and transcriptomic analyses of V. anguillarum grown under optimal immunogenic conditions revealed 323 upregulated genes, of which 211 were high-antigenicity proteins suitable for subunit vaccines. This study provides critical knowledge for effective vaccine formulation against V. anguillarum and identifies potential antigens for subunit vaccine development. • A model for systemic V. anguillarum infection and vaccine testing in lumpfish was developed. • V. anguillarum bacterins, prepared under iron-limited conditions with 2 % NaCl at 28 °C, provided the highest immune protection in lumpfish. • V. anguillarum bacterins prepared from cultures grown at 15 °C did not confer protection to the immunized lumpfish. • Reverse vaccinology and transcriptomic analysis of V. anguillarum grown under optimal immunogenicity conditions revealed 211 genes encoding high-antigenicity proteins. • These results indicated that V. anguillarum growth conditions significantly influence vaccine effectiveness.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".