A248 ETHANOL-INDUCED MUCOSAL IRRITATION DISRUPTS LOCAL AND SYSTEMIC PROTECTIVE EFFECTS OF NON-INVASIVE TRANSCUTANEOUS AURICULAR VAGUS NERVE STIMULATION (TAVNS)
Bibliographic record
Abstract
Abstract Background The integrity of the mucosal barrier is vital for gastrointestinal homeostasis. Previously, we demonstrated that non-invasive transcutaneous auricular vagus nerve stimulation (taVNS) decreases inflammatory and pro-apoptotic markers while increasing anti-inflammatory markers in homeostatic conditions. However, its effectiveness in case of slight mucosal irritation remains unclear. Aims To assess whether slight mucosal irritation affects taVNS efficacy in modulating pro- and anti-inflammatory cytokines and apoptosis-related markers in the colon and spleen. Methods Male C57BL/6 mice were given a single intrarectal dose of 30% ethanol (ETOH group, 100 µl), while the control (CTRL) group received no ethanol. Preventive taVNS (10V, 20Hz & 500us, 10min/day) or no stimulation (clipping only) began one day before ethanol injection and continued for four days. Colonic and splenic levels of interleukin (IL)-10, tumor growth factor (TGF)-β, tumor necrosis factor (TNF)-α, pro-apoptotic markers Bcl-2-associated X protein (BAX), Bcl-2 homologous antagonist killer (BAK1), caspase8, and pro-survival BCL2 associated agonist of cell death (BAD) were measured using qRT-PCR and ELISA. Results taVNS significantly increased IL-10 mRNA expression (p<0.0001) and both mRNA (p<0.015) and protein (p<0.0001) levels of TGF-β in CTRL conditions, with no changes in the ETOH group. Mice demonstrated unchanged IL-10 protein levels in the colon in the presence or absence of taVNS. However, in CTRL conditions, taVNS downregulated BAX (p<0.0001), BAK1(p<0.0001), and caspase8 (p<0.0002) mRNA expression significantly, with no effect in the ETOH group. Additionally, BAD (p<0.0001) mRNA expression increased significantly in CTRL conditions but remained unchanged in the ETOH group. In the spleen, taVNS decreased TNF-α (p<0.04) and increased TGF-β (p<0.0007) protein levels in CTRL conditions without impacting the ETOH group. Conclusions Our findings demonstrated that taVNS successfully regulated systemic and local cytokines and apoptosis-related markers in control mice. However, a slight irritation induced by ethanol abolished its effects, indicating that ethanol associated with a slight mucosa irritation can modify the taVNS outcome. Funding Agencies CIHR
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.003 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".