Examination of the role of the rumen in zinc metabolism
Bibliographic record
Abstract
Three experiments were conducted to characterize Zn appearance and disappearance in the rumen, rumen epithelial Zn importers and exporters, the directionality of Zn movement, and the effect of supplemental Zn on rumen epithelial structure. In experiments 1 and 2, ruminally fistulated steers (n = 4) weighing 736 ± 23.3 kg were used in a crossover experiment with 2 dietary Zn concentrations (ZINC; 0 [CON] or 120 mg supplemental Zn/kg DM [Zn120], from ZnSO4) in a 45% forage, 55% concentrate diet (experiment 1) or a 92.5% concentrate and 7.5% forage diet (experiment 2) to assess Zn appearance and disappearance in the rumen using the washed reticulo-rumen technique (WRR). Experiment 3 used 20 individually housed Hampshire crossbred wether lambs (35.1 ± 4.57 kg) fed for 60-d to evaluate the impact of dietary grain and Zn concentrations on ruminal epithelium Zn concentration, Zn transporters, and proteins integral to epithelial integrity. This experiment was a 2 × 2 factorial, with 2 diet types (DIET; 45% forage and 55% concentrate [HF] or 7.5% forage and 92.5% concentrate [HG]), and 2 supplemental Zn treatments (ZINC; 0 mg [CON] or 120 mg [ZN] supplemental Zn/kg diet DM) from ZnSO4. In experiments 1 and 2, ZINC did not affect the rate of disappearance (k) or plateau (B) digestive model parameters for buffer Zn (P ≥ 0.34) but ruminal Zn disappearance was numerically greater in experiment 2. In experiment 3, there was a DIET × ZINC effect (P = 0.05) where HG + ZN had the greatest rumen epithelial Zn, and HF + ZN had the least. There was an interaction between DIET and ZINC on papillae length (P = 0.05), where papillae length was shorter in HF than HG, and within HG, ZN animals had shorter papillae than CON (P = 0.01). A DIET × ZINC effect was noted for ZnT1 RNAscope score (P = 0.01) within the stratum spinosum layer where HG + CON wethers had greater mean ZnT1 RNAscope score than other treatments (P ≤ 0.006). Ruminal Claudin-7 protein expression was lesser in HF (P = 0.01) and tended to be lesser in ZN (P = 0.09). Overall, these experiments highlight the dynamic nature of ruminal Zn metabolism and are supporting evidence for the relationship between ruminal health and dietary grain and Zn concentrations.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".