Vitrification of porcine intact femoral condyle through different protocol lengths and loading strategies
Bibliographic record
Abstract
Cryopreservation of articular cartilage (AC) allografts is a potential candidate to solve the shortage of fresh osteochondral allografts to treat large AC defects. This technique requires optimization to address issues related to the actual process of cryopreservation and the translation of the technology to tissue banks. In the present study, we compared different vitrification protocols consisting of multiple cryoprotective agent (CPA) loading timings and loading strategies to achieve successful vitrification-rewarming protocols for porcine intact femoral condyles. Porcine medial femoral condyles (n = 6) were used to test 5 vitrification protocols differing in lengths of time and the method of CPA loading. The effects of these protocols on post-warming normalized chondrocyte viability, metabolic activity, and tissue cracking frequency were documented. We demonstrated that all 5 CPA loading-vitrification protocols resulted in high normalized chondrocyte viability (>88 % of Fresh control) post vitrification in porcine intact femoral condyles. Moreover, the Room Temperature Gel (RG) protocol showed high viability and metabolic activity of chondrocytes post-warming, and also decreased cracking frequency in AC after rewarming of vitrified intact femoral condyles. To facilitate translation of this technology to tissue banks, we showed that by shortening (total time of 15 min in Protocol RG) or combining our established Protocol 8 (430 min) with a hydrogel, including use of cryobags in liquid nitrogen vapour as the conventional storage method in tissue banks, we could introduce two reproducible protocols for clinical applications involving vitrified osteochondral tissue.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".