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Record W4408006873 · doi:10.1101/2025.02.26.24319604

Development and first-in-human CAR T therapy against the pathognomonic MiT-fusion driven protein GPNMB

2025· preprint· en· W4408006873 on OpenAlexaff
Franz J. Zemp, Zackariah Breckenridge, Hyojin Song, Hongrui Liu, Yeon Suh, Louisa Guignard, Joanna Pyczek, Cini Mathew John, Laura Mah, Jahanara Rajwani, Taye Louie, Danyel Evseev, Madison Turk, Sacha Benaoudia, Victor Naumenko, Varsha Thoppey Manoharan, Mobina Kazemi Mehrabadi, Geneece N.Y. Gilbert, Kiran Narta, Aaron Gilmour, Ted Verhey, Hayley M. Todesco, Katalin Osz, Bo‐Young Ahn, Ana Bogossian, Tarek A. Bismar, Daniel Y.C. Heng, Marston Lineham, Haley Pedersen, Paul E. Gordon, John B. McIntyre, John MacGregor, Kathy Brodeur‐Robb, Travis Ogilvie, Patrick Schöffski, Agnieszka Woźniak, Robert A. Holt, John Bell, Donna L. Senger, Victor Lewis, Michael J. Monument, Kyle Potts, Kiril Trpkov, Kevin A. Hay, Jennifer Quizi, Jennifer A. Chan, Ramy Saleh, Jan Willem Henning, Nicole L. Prokopishyn, A. Sorana Morrissy, Mona Shafey, Douglas J. Mahoney

Bibliographic record

VenuemedRxiv · 2025
Typepreprint
Languageen
FieldMedicine
TopicCAR-T cell therapy research
Canadian institutionsUniversity of OttawaMcGill UniversityUniversity of British ColumbiaQuest University CanadaUniversity of Calgary
Fundersnot available
KeywordsPathognomonicFusion proteinFusionMedicineRecombinant DNAChemistryPhilosophyBiochemistryInternal medicineDisease

Abstract

fetched live from OpenAlex

Abstract CAR T therapy for solid tumors is limited by a lack of safe and uniformly expressed cell-surface targets. Here, we identify the MiT fusion-driven protein GPNMB as being highly, homogeneously, and stably expressed in primary and relapsed translocation-positive alveolar soft part sarcoma (ASPS) and renal cell carcinoma (tRCC). We developed a GPNMB-targeting CAR T therapy called GCAR1 that shows activity against patient-matched cells, organoids and xenograft models. First-in-human treatment of a patient with metastatic ASPS was well tolerated and generated stable disease until 6 months, with many non-target lesions resolving post-treatment. A polyclonal population of GCAR1 cells expanded in blood and were detectable until 6 months. Spatial transcriptomics revealed multiple immunosuppressive niches in proximity to T cells infiltrating a treatment-resistant lesion, and PDL1 blockade showed synergy with GCAR1 in a xenograft model. Our data provide clinical evidence for treating solid tumors with CAR T cells targeting a surface protein driven by an oncogenic gene fusion.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.007

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.037
GPT teacher head0.312
Teacher spread0.275 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations3
Published2025
Admission routes1
Has abstractyes

Explore more

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