Changes in motor unit conduction velocity after unilateral lower limb suspension and active recovery correlate with muscle ion channel gene expression
Bibliographic record
Abstract
Abstract The effects of muscle disuse on the propagation of action potentials along muscle units, a key process for effective muscle activation and force production, remain poorly understood. This study aimed to investigate changes in action potential propagation and to identify biological factors influencing these changes following unilateral lower limb suspension (ULLS) and active recovery (AR). Eleven young male participants underwent 10 days of ULLS followed by 21 days of AR based on resistance exercise. Maximal force of the knee extensor muscles (MVC), High-Density surface EMG recordings and muscle biopsies of the vastus lateralis muscle were collected before ULLS, after ULLS and after AR. EMG recordings collected during submaximal isometric contractions were decomposed to estimate single motor unit conduction velocity (MU CV). Muscle biopsies were used to measure muscle fibre diameters via histochemical analysis and ion channel transcriptomic profiles via mRNA-sequencing. MVC decreased after ULLS by 29% and fully recovered after AR. MU CV decreased after ULLS and fully recovered, up to exceeding baseline values after AR. Muscle fibre diameters did not change across the interventions and showed no correlation with MU CV. Conversely, a feature importance analysis revealed that mRNA expression levels of specific ion channel genes, particularly those involved in K + transport, correlate with MU CV at baseline and across the interventions. This study highlights the crucial role of K + ion channels in influencing MU CV in humans, offering new insights into MU CV modulation and the mechanisms of muscle force changes after disuse and active recovery. Key points Muscle disuse, such as in unilateral lower limb suspension, leads to a decrease in motor unit conduction velocity (MU CV), a critical factor for muscle activation and force production. Active recovery through resistance exercise results in the full recovery of MU CV, even exceeding baseline levels. Muscle fibre diameters do not change significantly after limb suspension or active recovery and show no correlation with MU CV. Conversely, ion channel mRNA expression, particularly of those related to K + transport, correlates with MU CV and its changes following disuse and recovery. These findings highlight K + ion channels as a key factor in regulating MU CV in humans and provide new molecular determinants of the changes in muscle force after disuse and recovery.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".