Characterization of a Choline-Gated Chloride Channel (LGC-40) from Haemonchus contortus Highlights a Novel Cholinergic Binding Site
Bibliographic record
Abstract
Nematode cys-loop ligand-gated chloride channels are important targets for anthelmintic drugs as many subtypes are not present in mammals. We report the isolation and functional characterization of a novel member of the cys-loop ligand-gated chloride channel family (Hco-LGC-40) from the parasitic nematode Haemonchus contortus. Electrophysiological analysis of the channel expressed in Xenopus oocytes revealed that it responds to both acetylcholine and choline with EC50 values in the low micromolar range. Hco-LGC-40 can also associate with a member of a separate family of acetylcholine-gated chloride channels, Hco-ACC-1, to produce a heteromeric channel with a lower sensitivity to acetylcholine. In silico analysis reveals several residues that may play a role in forming a unique cholinergic binding pocket. Overall, these results suggest that choline may act as a neurotransmitter by binding to cys-loop receptors in parasitic nematodes.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".