The <scp>PP2A</scp> ‐ <scp>B56</scp> Binding Site <scp>LxxIxE</scp> Contributes to Asp‐Mediated Spindle Pole Stability
Why this work is in the frame
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Bibliographic record
Abstract
ABSTRACT The organization of microtubules into a mitotic spindle is critical for animal cell proliferation and involves the cooperation of hundreds of proteins whose molecular roles and regulation are not fully understood. The protein product of the Drosophila gene abnormal spindle , Asp, is a microtubule‐associated protein required for correct mitotic spindle formation. To better understand the contribution of Asp to microtubule organization during spindle formation, we reverse‐engineered flies to express a version of Asp (Asp LIE ), predicted to have lost its ability to bind the phosphatase trimer PP2A‐B56. We demonstrated that the Asp LIE mutation reduced an interaction with the Drosophila PP2A‐B56 regulatory subunit Widerborst (Wdb), as well as other proteins with known roles in spindle formation. Asp LIE flies exhibited less robust microtubule minus‐end cohesion at neural stem cell spindle poles, which was accompanied by a substantial developmental delay but no microcephaly. Predictive structural modeling suggests that the presence of Wdb alters the conformation of an Asp interaction with a tubulin dimer in a manner similar to that of the Asp LIE mutation. Protein localization in the Drosophila embryo, in addition to in vitro microtubule organization experiments, suggests that a role of PP2A may be to prevent Asp from contributing to microtubule cross‐linking at spindle microtubule plus ends. Together, these findings add new insights to mechanisms underlying microtubule organization within the mitotic spindle.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.007 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it