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Record W4408556965 · doi:10.1016/j.xpro.2025.103681

Protocol for assessing distances in pathway space for classifier feature sets from machine learning methods

2025· article· en· W4408556965 on OpenAlexaff
Bahar Tercan, Victor H Apolonio, Vinicius S Chagas, Christopher K. Wong, Jordan Lee, Christina Yau, Christopher C. Benz, Joshua M. Stuart, B. Karlberg, Kyle Ellrott, Jasleen Grewal, Steven J.M. Jones, A. Gordon Robertson, Jean C. Zenklusen

Bibliographic record

VenueSTAR Protocols · 2025
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMachine Learning in Bioinformatics
Canadian institutionsCanada's Michael Smith Genome Sciences Centre
FundersFundação AraucáriaConselho Nacional de Desenvolvimento Científico e TecnológicoCoordenação de Aperfeiçoamento de Pessoal de Nível SuperiorNational Cancer InstituteNational Institutes of HealthFundacion Araucaria
KeywordsComputer scienceClassifier (UML)Artificial intelligenceProtocol (science)Machine learningFeature vectorPattern recognition (psychology)Medicine

Abstract

fetched live from OpenAlex

As genes tend to be co-regulated as gene modules, feature selection in machine learning (ML) on gene expression data can be challenged by the complexity of gene regulation. Here, we present a protocol for reconciling differences in classifier features identified using different ML approaches. We describe steps for loading the PathwaySpace R package, preparing input for analysis, and creating density plots of gene sets. We then detail procedures for testing whether apparently distinct feature sets are related in pathway space. For complete details on the use and execution of this protocol, please refer to Ellrott et al. 1 • Protocol to test whether distinct gene sets reflect related biology • Steps for building a pathway space for graph- or network-based distance analysis • Instructions for calculating a pathway distance metric for any pair of gene sets • Guidance on exploring relationships between gene sets in pathway space Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. As genes tend to be co-regulated as gene modules, feature selection in machine learning (ML) on gene expression data can be challenged by the complexity of gene regulation. Here, we present a protocol for reconciling differences in classifier features identified using different ML approaches. We describe steps for loading the PathwaySpace R package, preparing input for analysis, and creating density plots of gene sets. We then detail procedures for testing whether apparently distinct feature sets are related in pathway space.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Protocol · Consensus signal: Protocol
Teacher disagreement score0.872
Threshold uncertainty score0.950

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.036
GPT teacher head0.442
Teacher spread0.406 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designNot applicable
Domainnot available
GenreProtocol

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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