Single genome amplification and molecular cloning of HIV-1 populations in acute HIV-1 infection: implications for studies on HIV-1 diversity and evolutionary rate
Bibliographic record
Abstract
ABSTRACT Background Human immunodeficiency virus type 1 (HIV-1) is one of the fastest evolving human pathogens. Understanding transmission, within-host adaptation, and evolutionary dynamics are pivotal for development of interventions and vaccines. HIV-1 infection is generally caused by one single transmitted founder virus (TFV), and TFV sequences have typically been obtained using single genome amplification (SGA). However, suboptimal sample quality can result in sequencing failures, representing non-trivial losses considering the scarcity of acute HIV-1 infection (AHI) samples. Sequencing failures may be mitigated by molecular cloning (MC), a method that can be less vulnerable to sample quality but more susceptible to PCR errors. Here, we explore the feasibility of supplementing SGA with MC data using samples from clinical and research cohorts to determine whether sequence diversity and evolutionary rate estimates are comparable between the two techniques. Methods Participants were enrolled in an East African research cohort from the International AIDS Vaccine Initiative 2006-2011 or a clinical cohort from Sweden (1983-2011). SGA and MC sequencing were done on the HIV-1 env V1-V3 region (approximately 940 base pairs). Within-host sequence diversity was determined from maximum likelihood phylogenetic trees and evolutionary rate by Bayesian phylogenetic analysis. Highlighter and Poisson-Fitter tools, Hamming distances, and assessment of star phylogenies were used to quantify TFVs. Results Participants with AHI (N=100, median age 30.3 years, 15% female) were included, contributing 350 samples from four longitudinal time points 10-540 days post infection. SGA succeeded on 90% of research cohort and 48% of clinical cohort samples. Comparative analysis of linked SGA and MC data from 10 samples indicated that approximately eight sequences were necessary for diversity estimates. Consistently higher sequence diversity was observed among MC relative to SGA sequences (mean±SD 0.009±0.007 and 0.006±0.006 substitutions/site, p<0.001), whereas evolutionary rates were similar between the two methods (mean±SD 0.014±0.006 vs. 0.014±0.009 substitutions/site/year, p=0.673). Five participants with visits within 45 days post infection were eligible for TFV quantification and all found to have one TFV using both MC and SGA data. Conclusion MC data is a suitable supplement for SGA-based studies to preserve the value of precious samples for evolutionary rate but not sequence diversity analysis.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.022 | 0.055 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.002 | 0.002 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.002 | 0.002 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".