Fructilactobacillus frigidiflavus sp. nov., a pigmented species, and Levilactobacillus lettrarii sp. nov., a propionate-producing species isolated from sourdough
Bibliographic record
Abstract
The sourdough isolates FUA3702, FUA3912 and FUA3913T, as well as FUA3695T and FUA3914, could not be identified to known species of the Lactobacillaceae. The 16S rRNA gene sequences of FUA3702 and FUA3913, FUA3695 and FUA3914 were>99% identical to Fructilactobacillus sanfranciscensis and Levilactobacillus lanxiensis, respectively. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values of strain FUA3913T when compared to Fl. sanfranciscensis were 83.67 and 26.60%, respectively. In addition, strains FUA3702, FUA3912 and FUA3913T produce different levels of a yellow C30 carotenoid, but pigmentation has not been described in Fl. sanfranciscensis. The ANI and dDDH values of FUA3695T and FUA3914 when compared to Lv. langxiensis were 95.22 and 61.20%, respectively. In addition, FUA3695 and FUA3914 convert lactate to 1,2-propanediol and further to propionate. The conversion of lactate to propionate by a single strain has not been documented for any of the species in the Lactobacillaceae. Based on the genomic and physiological characteristics, we proposed the novel species Fructilactobacillus frigidiflavus sp. nov. FUA3913T (=DSM 118650T=LMG 33758T) and Levilactobacillus lettrarii sp. nov. FUA3695T (=DSM 118651T=LMG 33759T).
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.001 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".