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Record W4408853456 · doi:10.1038/s41586-025-08746-0

Connectome-driven neural inventory of a complete visual system

2025· article· en· W4408853456 on OpenAlexaff
Aljoscha Nern, Frank Loesche, Shin-ya Takemura, Laura E. Burnett, Marisa Dreher, Eyal Gruntman, Judith Hoeller, Gary B. Huang, Michał Januszewski, Nathan C Klapoetke, Sanna Koskela, Kit D. Longden, Zhiyuan Lu, Stephan Preibisch, Wei Qiu, Edward M. Rogers, Pavithraa Seenivasan, Arthur Zhao, John Bogovic, Brandon S Canino, Jody Clements, Michael Cook, Samantha Finley-May, Miriam A Flynn, Imran Hameed, Alexandra M. C. Fragniere, Kenneth J. Hayworth, Gary Patrick Hopkins, Philip M. Hubbard, William T. Katz, Julie Kovalyak, Shirley A Lauchie, Meghan Leonard, Alanna Lohff, Charli Maldonado, Caroline Mooney, Nneoma Okeoma, Donald J. Olbris, Christopher Ordish, Tyler Paterson, Emily M Phillips, Tobias Pietzsch, Jennifer Rivas Salinas, Patricia K. Rivlin, Philipp Schlegel, Ashley L Scott, L. A. Scuderi, Satoko Takemura, Iris Talebi, Alexander Thomson, Eric T. Trautman, Lowell Umayam, Claire Smith, John J Walsh, C. Shan Xu, Emily A Yakal, Tansy Yang, Ting Zhao, Jan Funke, Reed George, Harald F. Hess, Gregory S.X.E. Jefferis, Christopher Knecht, Wyatt Korff, Stephen M. Plaza, Sandro Romani, Stephan Saalfeld, Louis K. Scheffer, Stuart Berg, Gerald M. Rubin, Michael B. Reiser

Bibliographic record

VenueNature · 2025
Typearticle
Languageen
FieldNeuroscience
TopicNeurobiology and Insect Physiology Research
Canadian institutionsThe Scarborough HospitalUniversity of Toronto
FundersNational Institute of General Medical SciencesWellcome Trust
KeywordsConnectomeComputer scienceConnectomicsSet (abstract data type)VisualizationNeuroscienceVisual processingArtificial intelligencePattern recognition (psychology)BiologyFunctional connectivityPerception

Abstract

fetched live from OpenAlex

Abstract Vision provides animals with detailed information about their surroundings and conveys diverse features such as colour, form and movement across the visual scene. Computing these parallel spatial features requires a large and diverse network of neurons. Consequently, from flies to humans, visual regions in the brain constitute half its volume. These visual regions often have marked structure–function relationships, with neurons organized along spatial maps and with shapes that directly relate to their roles in visual processing. More than a century of anatomical studies have catalogued in detail cell types in fly visual systems 1–3 , and parallel behavioural and physiological experiments have examined the visual capabilities of flies. To unravel the diversity of a complex visual system, careful mapping of the neural architecture matched to tools for targeted exploration of this circuitry is essential. Here we present a connectome of the right optic lobe from a male Drosophila melanogaster acquired using focused ion beam milling and scanning electron microscopy. We established a comprehensive inventory of the visual neurons and developed a computational framework to quantify their anatomy. Together, these data establish a basis for interpreting how the shapes of visual neurons relate to spatial vision. By integrating this analysis with connectivity information, neurotransmitter identity and expert curation, we classified the approximately 53,000 neurons into 732 types. These types are systematically described and about half are newly named. Finally, we share an extensive collection of split-GAL4 lines matched to our neuron-type catalogue. Overall, this comprehensive set of tools and data unlocks new possibilities for systematic investigations of vision in Drosophila and provides a foundation for a deeper understanding of sensory processing.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.007

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0020.002
Science and technology studies0.0000.000
Scholarly communication0.0010.001
Open science0.0000.001
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.027
GPT teacher head0.334
Teacher spread0.308 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations50
Published2025
Admission routes1
Has abstractyes

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