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Record W4409246424 · doi:10.1038/s41597-025-04921-0

Whole Genome Sequence of the gut commensal protist Tritrichomonas musculus isolated from laboratory mice

2025· article· en· W4409246424 on OpenAlexafffund
Eliza V.C. Alves‐Ferreira, Madeline R. Galac, Hernán Lorenzi, Margaret C.W. Ho, Erick T. Tjhin, Ana Popovic, John Parkinson, Michael E. Grigg

Bibliographic record

VenueScientific Data · 2025
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenomics and Phylogenetic Studies
Canadian institutionsUniversity of TorontoHospital for Sick Children
FundersNHLBI Division of Intramural ResearchCanadian Institutes of Health ResearchU.S. Department of Health and Human ServicesNational Institutes of HealthGovernment of CanadaNational Institute of Allergy and Infectious DiseasesDivision of Intramural Research, National Institute of Allergy and Infectious Diseases
KeywordsBiologyGenomeGeneAxenicGeneticsGiardiaModel organismProtistEntamoebaGiardia lambliaWhole genome sequencingComputational biologyEntamoeba histolyticaBacteria

Abstract

fetched live from OpenAlex

Tritrichomonas musculus is a commensal protist colonizing the large intestine of laboratory mice. Parasite colonization reshapes the gut microbiome and modulates mucosal immunity. This parasite is refractory to axenic culture. In order to facilitate functional genomic investigations we assembled a 193.49 Mbp high quality reference genome from FACS-purified parasites recovered from monocolonized mice using an integrated approach that combined long-read (PacBio and Oxford Nanopore) sequencing technologies for the draft genome assembly. The genome assembled into 756 contigs and RNA-Seq data was used to support the gene models for 46,131 annotated genes. Of these, 24,215 genes had an InterPro, Enzyme Commission and/or a Gene Ontology annotation. BUSCO analyses established that 53% of the genome annotations matched with available BUSCO genes in the eukaryote_odb10 database. This high quality reference genome will serve as a valuable resource to develop a metabolic and genetic model to grow T. musculus axenically and study genes relevant to its biology, life cycle transmission, and pathogenesis.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.901
Threshold uncertainty score0.354

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.002
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.025
GPT teacher head0.270
Teacher spread0.245 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2025
Admission routes2
Has abstractyes

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