Endocrine regulation of feeding in non-transgenic and transgenic fluorescent orange tiger barb (Puntigrus tetrazona)
Bibliographic record
Abstract
• Non transgenic and transgenic orange tiger barbs had similar levels of feeding. • Fasting decreased expression of anorexigenic factors and increased that of orexigenic factors. • Increasing temperatures increased food intake in both non transgenic and orange fish. • Decreases and increases in temperature affected the expression of appetite regulating peptides in both strains. • Fasting- and temperature- induced changes in brain expression were more pronounced in transgenic fish. Tiger barbs are popular tropical aquarium fish but despite their economic importance, nothing is known about their feeding physiology, in particular their endocrine regulation of feeding. The tiger barb has also been used to make genetically modified fluorescent fish but the influence of this genetic modification on their physiology is poorly understood. In this study, we submitted both non-transgenic (NT) and transgenic fluorescent orange (T) fish to 2 weeks of fasting or different temperatures (20, 25 and 30 °C) and assessed food intake and the expression of appetite regulators in brain, intestine and liver. Fasting had no effect on appetite regulators in the intestine, and decreased liver leptin expression in NT fish only. Fasting caused an overall increase and decrease in brain orexigenic and anorexigenic factors, respectively. The nature of peptides affected by this response differed between strains (MCH, ghrelin, POMCb in both NT and T, orexin in NT only, CRF and CCK in T only). In both T and NT fish, increasing temperatures increased food intake. Temperature affected the expression of most of the peptides examined, but the effects differed between the two fish strains. A shift from 25 to 20 °C increased hepatic leptin in NT and T, and intestine ghrelin in NT and had no effect on brain expression. A shift from 25 to 30 °C did not affect intestine or liver expressions, increased orexin, MCH and CRF brain expression in NT and T, and increased POMCb and CCKa expressions in T. Our study presents new information on the endocrine regulation of feeding in tiger barb, and provides insights on how transgenesis might affect feeding physiology of fish.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".