<i>LncRNA Dio3os</i> regulates neighboring gene <i>Dio3</i> and impacts osteogenesis in trans
Bibliographic record
Abstract
Consumptive hypothyroidism, a rare pediatric disorder, arises from aberrant Dio3 expression, which inactivates thyroid hormones and disrupts skeletal development. This study investigates the regulatory role of the long non-coding RNA Dio3os, which activates Dio3 in cis and suppresses osteoblast differentiation in trans. We focused on mouse Dio3os variant 203 and human variants 203 and 205. Chromatin accessibility and histone modification analyses revealed higher chromatin openness and active histone H3 modifications at the Dio3os promoter and exons 2 and 3, followed by increased RUNX2 binding with reduced histone H3 modifications during maturation. Dio3os expression is enhanced by HDAC1/2, HIF1a, and thyroid hormones, but repressed by BMP2, TGFb, Runx2, and Brg1. Overexpression of Dio3os upregulated Dio3 while downregulating osteogenic markers. CRISPR-mediated deletion of Dio3os exons or premature intronic polyadenylation suppressed Dio3 and restored osteogenic gene expression. RNA-seq and ATAC-seq confirmed enhanced thyroid hormone-responsive osteoblast gene activity in Dio3os-CRISPR-knockout cells. These findings reveal Dio3os as a key regulator of thyroid hormone metabolism and bone formation, presenting a novel target for treating skeletal abnormalities in early childhood hypothyroidism.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.004 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".