Plasmid <scp>pPNptGreen</scp> Expression of Green Fluorescent Protein in <scp> <i>Pseudomonas chlororaphis</i> </scp> Strain <scp>S1Bt23</scp> Abrogates Biocontrol Activity Against <scp> <i>Pythium ultimum</i> </scp>
Bibliographic record
Abstract
Pseudomonas chlororaphis is a highly effective plant root coloniser and biocontrol agent. To monitor the colonisation of tomato and canola roots, P. chlororaphis S1Bt23 was transformed with the pPNptGreen plasmid encoding for green fluorescent protein (S1Bt23-GFP). Seedling roots inoculated with S1Bt23-GFP were examined after 2 and 5 h using confocal laser fluorescence microscopy. Roots exposed to S1Bt23-GFP showed pronounced biofilm formation around the root surface, and fluorescing cells were localised in the epidermis and metaxylem after 2 and 5 h of inoculation, respectively. The canola roots also showed upward active translocation of the S1Bt23-GFP cells in xylem vessels in real time. S1Bt23-GFP was also evaluated for antagonistic activity against Pythium ultimum. While S1Bt23 WT exhibited 65.70%-71.4% inhibition of radial growth of Py. ultimum, the S1Bt23-GFP strain did not demonstrate any antagonistic effects. Thin layer chromatography and liquid chromatography mass spectrometry analyses of culture extracts of S1Bt23-GFP did not detect phenazines or pyrrolnitrin, antifungal metabolites identified in S1Bt23 wild type. Expressions of phenazine and pyrrolnitrin genes showed no differences in S1Bt23-GFP and wild type. This suggests that the abrogation of these metabolites occurred post-transcriptionally, probably due to a high cellular molecular load in GFP production. This could negatively impact the ecological fitness of S1Bt23-GFP.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".