DREADD-mediated inhibition of anterior retrosplenial cortex: Effects on novelty recognition of objects, locations, and object-in-place associations in male and female Long Evans rats
Bibliographic record
Abstract
Previous research suggests ionotropic glutamate receptors in anterior retrosplenial cortex (aRSC) are important for short-term (1-hour) object-in-place (OiP) novelty recognition, indicated by enhanced interaction with novel object-location pairs during OiP test phases. Here, male and female rats were repeatedly tested in three 1-hour delay novelty recognition tests: object recognition (OR), object location (OL), and OiP. Prior to behavioral testing, control (AAV5-CaMKIIα-mCherry) or active (AAV5-CaMKIIα-hM4D(Gi)-mCherry) viral vectors were bilaterally infused into the aRSC of male (8 control, 13 active) and female (8 control, 13 active) Long Evans rats, enabling selective inhibition of aRSC neurons with the hM4D agonist Compound 21 (C-21). Following recovery from surgery, rats were repeatedly tested in recognition tests following injection of either saline or C-21 (1.0 mg/kg; i.p.) ∼45-min prior to test phases (6 tests/rat). Analyses of exploration times indicated that total object interaction times did not differ between phase, sex, or treatment. Further analyses revealed that C-21 treatment of rats infused with the active vector reduced novelty recognition in the OR test yet had no influence in the OL test, regardless of sex. Interestingly, C-21 also reduced novelty recognition in OiP recognition test phases, an effect only observed in male rats infused with the active vector. Findings highlight a nuanced influence of aRSC neurons in supporting novelty recognition which varies by sex and type of stimuli assayed.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".