Genome-wide identification of Morus notabilis Aquaporin gene family and differential expression of plasma membrane intrinsic proteins in response to Ralstonia pseudosolanacearum infection
Bibliographic record
Abstract
BACKGROUND: Aquaporins (AQPs) play crucial roles in plant water transport, growth and development, and response to biotic and abiotic stresses. Despite their essential functions, the role of AQPs in mulberry trees (Morus L.) during Ralstonia pseudosolanacearum infection remains largely unknown. RESULTS: In this study, we performed a genome-wide identification and comprehensive characterization of AQP genes in Morus notabilis. A total of 26 AQP genes were identified and classified into four subfamilies: NIP, PIP, SIP and TIP. Furthermore, detailed analyses were conducted on gene structures, protein physicochemical properties, transmembrane domains, phylogenetic relationships, and subcellular localization. Cis-acting element analysis showed that MnAQP genes were mainly involved in hormone, light and stress response. Tissue-specific expression analysis demonstrated that the PIP1 subfamily displayed significantly higher transcript abundance in root tissues relative to leaves, while the PIP2 subfamily maintained relatively stable expression patterns across various tissue types. In addition, following R. pseudosolanacearum inoculation, the expression levels of PIP genes were significantly upregulated in the roots, stems, and leaves of mulberry seedlings. These findings indicate that MnPIP genes play a crucial role in responding to R. pseudosolanacearum infection. CONCLUSIONS: This study provides the comprehensive characterization of the AQP gene family in mulberry, clarifying the composition and diversity. Our findings establish a solid foundation for further understanding the function roles of MnPIPs following R. pseudosolanacearum infection in mulberry trees. CLINICAL TRIAL NUMBER: Not applicable.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".