Abstract CT016: First-in-human (FIH) phase 1 trial of the oral first-in-class covalent Werner helicase (WRN) inhibitor RO7589831 in patients with microsatellite instable (MSI) and/or mismatch repair deficient (dMMR) advanced solid tumors
Bibliographic record
Abstract
Abstract Background: WRN is an enzyme critical for DNA repair and genome stability and is a promising synthetic lethal target for MSI cancers. 40-70 % of patients (pts) with MSI/dMMR solid tumors do not respond to immune checkpoint inhibitors (ICI) or develop resistance, representing an unmet need in MSI cancers. RO7589831 is a novel first-in-class covalent, irreversible WRN inhibitor that induces dose-dependent DNA damage and tumor growth inhibition in MSI preclinical models, supporting its clinical evaluation in MSI pts. Methods: This open-label, multi-center, FIH study assesses the safety, pharmacokinetics (PK), pharmacodynamics (PD) and preliminary anti-tumor activity of RO7589831 in pts with MSI and/or dMMR advanced solid tumors (NCT06004245). The primary objective is to determine the maximum tolerated dose (MTD) and/or recommended Phase 2 dose (RP2D). 14 paired tumor biopsies were collected for translational analysis. Results: As of Nov 25, 2024, 44 pts (24 F, 20 M; median age 62y (32-77y)) were enrolled in six dose cohorts at RO7589831 daily doses from 150 mg to 2000 mg. Based on clinical first dose PK data (rapid absorption, mean Tmax 2.6 h [± 1.3h] and half-life 4.4 h [±1.8h], n=41), RO7589831 was dosed orally once (QD) or twice (BID) daily. Among the 44 pts (22 colorectal (CRC) and 22 non-CRC), median prior treatment lines were 3 (range 1-12); 89% had received ICIs. 35 pts had MSI tumors (18 CRC and 17 non-CRC). No dose-limiting toxicities (DLTs) have been reported and the MTD has not been reached. The most common treatment-emergent AEs across all doses were nausea (52.3%), diarrhea (34.1%), and vomiting (31.8%). Grade (G)3 treatment related AEs (TRAE) were nausea and increased AST/ALT in 2 pts each (4.3%) as well as fatigue and anemia in 1 pt each (2.1%). No G4 or worse TRAEs were observed. Of the efficacy evaluable MSI pts (n= 32), four RECISTv1.1 partial responses (PRs) (2 confirmed and 2 ongoing unconfirmed PRs) were observed in post-ICI-treated MSI CRC (n=1), ovarian (n=1), and endometrial cancer (n=2) for up to 9.5+ mths. Disease control rate (DCR) was 68.8% (95%CI 51.13, 86.37) and 20 (62.5%) pts achieved RECISTv1.1 stable disease (SD). 48.4% (15/31) of FDG-PET scan evaluable pts across all dose levels showed metabolic responses to RO7589831. All pts with RECIST PR showed pronounced metabolic responses (-50% to -90%). ctDNA molecular responses were observed in 2/2 pts with RECISTv1.1 PR, 8/9 pts with SD and 0/5 pts with disease progression. Conclusions: RO7589831 is generally safe and well tolerated, with promising DCR, durable RECISTv1.1, FDG-PET metabolic and ctDNA molecular responses in pts with advanced MSI cancers, including ICI-treated pts, providing the first early clinical proof-of-concept for effectively drugging WRN. Dose optimization cohorts are ongoing to establish the RP2D. Citation Format: Timothy A. Yap, Natalie Cook, Elisa Fontana, Erica S. Tsang, Oliver Bechter, Marwan Fakih, Kristoffer S. Rohrberg, Emiliano Calvo, Elena M. Elez, Meredith Pelster, John H. Strickler, Ignacio Matos, Adam Sharp, Sophia Blake, David Dejardin, Stephen Fowler, Nina Henkel, Michael Hettich, Angelika Lahr, Ting Liu, Christophe Meille, Piergiorgio Pettazzoni, Denise Reeves, Barbara Romagnoli, Sotirios Sotiriou, Daniel Waterkamp, Yali Fu, Sophie Postel-Vinay. First-in-human (FIH) phase 1 trial of the oral first-in-class covalent Werner helicase (WRN) inhibitor RO7589831 in patients with microsatellite instable (MSI) and/or mismatch repair deficient (dMMR) advanced solid tumors [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2025; Part 2 (Late-Breaking, Clinical Trial, and Invited Abstracts); 2025 Apr 25-30; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2025;85(8_Suppl_2):Abstract nr CT016.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.002 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.003 |
| Insufficient payload (model declined to judge) | 0.008 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".