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Record W4409906957 · doi:10.1101/2025.04.28.25326545

REM sleep EEG slowing reflects brain cholinergic denervation in aging and Mild Cognitive Impairment

2025· preprint· en· W4409906957 on OpenAlexaff
Claire André, Marc‐André Bédard, Véronique Daneault, Rebekah Wickens, Jean‐Paul Soucy, Dominique Lorrain, Célyne Bastien, Carol Hudon, Nicola Andrea Marchi, Jacques Montplaisir, Nadia Gosselin, Julie Carrier

Bibliographic record

VenuemedRxiv · 2025
Typepreprint
Languageen
FieldNeuroscience
TopicSleep and Wakefulness Research
Canadian institutionsUniversité LavalMontreal Neurological Institute and HospitalUniversité du Québec à MontréalUniversité de SherbrookeUniversité de Montréal
Fundersnot available
KeywordsDenervationElectroencephalographyCholinergicNeuroscienceSleep (system call)Brain agingCognitive impairmentCognitionNeuroscience of sleepPsychologyAudiologyMedicineSlow-wave sleepInternal medicineComputer science

Abstract

fetched live from OpenAlex

Abstract Brain cholinergic denervation is among the earliest manifestations of Alzheimer’s disease, and rapid eye movement (REM) sleep alterations have also been described early in the course of the disease. While cholinergic activity supports cortical activation during REM sleep, direct evidence for a link between cholinergic degeneration and early REM sleep alterations in humans is still lacking. Here, we tested the long-standing hypothesis that early cholinergic denervation may be associated with REM sleep EEG slowing in older adults with and without Mild Cognitive Impairment. Twenty-four older participants (mean age: 71.29 ± 4.85 years; 58.33% women; 25% participants with amnestic Mild Cognitive Impairment) without dementia or moderate-to-severe obstructive sleep apnea underwent a night of in-laboratory polysomnography, comprehensive neuropsychological evaluation, structural MRI and molecular PET imaging with [ 18 F]-Fluoroethoxybenzovesamicol (FEOBV), known for its sensitivity to quantify brain cholinergic innervation. Voxel-wise multiple regressions assessed the associations between REM sleep characteristics (i.e., REM sleep percentage, relative theta power and EEG slowing ratios, defined as [delta + theta]/[alpha + beta] power) and FEOBV-PET standard uptake value ratio maps corrected for partial volume effects, controlling for sex. Given that FEOBV uptake was higher in women compared to men, we also performed exploratory sex-stratified analyses adjusted for age. Higher REM sleep EEG slowing over frontal and parietal derivations was significantly associated with cortical cholinergic denervation, notably in fronto-parietal areas and the medial temporal lobe ( P<0.005 level, combined with a cluster-level family-wise error correction). Exploratory sex-stratified analyses revealed that REM sleep EEG slowing was associated with cholinergic denervation in medial temporal regions in women, and neocortical regions in men. These findings provide the first direct in vivo evidence that REM sleep EEG slowing could represent a sensitive marker of cortical cholinergic denervation in older adults, prior to dementia onset. Thus, quantitative REM sleep EEG may constitute a promising marker for early diagnosis and disease-modifying interventions in Alzheimer’s disease.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.005

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.064
GPT teacher head0.359
Teacher spread0.295 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations3
Published2025
Admission routes1
Has abstractyes

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