Dental follicle stem cell-derived small extracellular vesicles ameliorate pulpitis by reprogramming macrophage metabolism
Bibliographic record
Abstract
Vital pulp therapy (VPT) is considered a conservative means of preserving the vitality and function of the dental pulp after injury. However, current VPT has unfavorable effects on inflamed pulp. Mesenchymal stem cell (MSC)-derived small extracellular vesicles (MSC-sEVs) show powerful immunomodulatory capacities and exert therapeutic effects on a variety of inflammatory diseases. However, whether MSC-sEVs ameliorate the inflammatory response and promote inflammatory pulp repair in pulpitis is largely unknown. In this study, we show that sEVs derived from dental follicle stem cells (typical dental MSCs, DFSC-sEVs) alleviate lipopolysaccharide-induced pulpitis in rats and enhance pulp repair by inducing M2 macrophage polarization. Mechanistically, heat shock protein 70 (HSP70) within DFSC-sEVs can be supplemented into lysosomes to directly protect lysosomal function and induce mitophagy to promote the degradation of depolarized mitochondria, thereby preprogramming inflammatory macrophages to commit to oxidative phosphorylation, which fuels M2 polarization. Furthermore, DFSC-sEVs also transfer antioxidant miRNAs, including miR-24-3p and let-7c-5p , to inhibit mitochondrial reactive oxygen species production, thereby indirectly stabilizing lysosomes to induce M2 macrophage generation. Our study reveals a promising immunotherapeutic potential of DFSC-sEVs for VPT in inflamed pulp and a novel role for DFSC-sEVs in inhibiting the macrophage inflammatory response by protecting lysosomes and inducing mitophagy-mediated metabolic shifts toward oxidative phosphorylation. • DFSC-sEVs show immunotherapeutic potential for vital pulp therapy in inflamed pulp. • DFSC-sEV-treated macrophages metabolically reprogram to OXPHOS, leading to anti-inflammatory phenotype. • DFSC-sEVs induce mitophagy by lysosomal protection, thereby preventing mitochondrial dysfunction in inflammatory macrophages. • HSP70 within DFSC-sEVs is required to protect lysosomes in inflammatory macrophages. • DFSC-sEVs transfer antioxidant miR-24-3p and let-7c-5p to synergetically protect lysosomal function.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".