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Record W4410344575 · doi:10.1371/journal.pone.0321690

A qualitative systematic review of barriers and facilitators to the implementation of community-based molecular diagnostics for infectious diseases

2025· review· en· W4410344575 on OpenAlexafffund
Jia Tong Song, Mai‐Lei Woo Kinshella, Jennifer Cochrane, Karen Mooder, K. Hassani, Michelle Dittrick, David A. Goldfarb

Bibliographic record

VenuePLoS ONE · 2025
Typereview
Languageen
FieldMedicine
TopicSARS-CoV-2 detection and testing
Canadian institutionsCommunity Based Research CentreBC Children's HospitalB.C. Women's Hospital & Health CentreChildren's & Women's Health Centre of British ColumbiaUniversity of British Columbia
FundersBC Children's Hospital
KeywordsUsabilityChecklistStaffingWorkloadMedicineHealth careMEDLINEThematic analysisPsychological interventionCritical appraisalNursingQualitative researchMedical educationPsychologyComputer sciencePathologyAlternative medicine

Abstract

fetched live from OpenAlex

BACKGROUND: Community-based molecular diagnostic testing for infectious diseases can bring equitable healthcare to resource-limited settings without hospital-based laboratories. Low-complexity molecular testing devices allow for unprecedented sensitivity and specificity for infectious disease diagnostics outside of a dedicated laboratory, which may facilitate timely initiation of therapeutics and other public health interventions in rural, remote and/or marginalized communities. OBJECTIVE: To identify barriers and facilitators to the implementation of community-based molecular testing. METHODS: A systematic search was conducted on MEDLINE Ovid, EMBASE, Web of Science, Cumulative Index to Nursing and Allied Health, Google Scholar, and reference lists. Original research that includes implementation of molecular testing systems at the community-level or at rural/remote health facilities with frontline healthcare workers and reports barriers and facilitators to implementation were included. Studies were assessed by the Critical Appraisal Skills Programme Qualitative Checklist and underwent inductive thematic analysis. The review protocol was registered to Prospero prior to conducting the review (CRD42023397800). RESULTS: A total of 6 studies were included in the review. We found three main themes present across all six included studies: infrastructure, usability, and staffing considerations. Infrastructure emerged as a critical determinant, with challenges ranging from physical space constraints to issues with reliable electricity and internet connectivity. The usability of testing devices, encompassing factors like ease of use and testing quality, also played a pivotal role. Staffing considerations, including workload, training, and attitudes, significantly influenced implementation outcomes. CONCLUSION: Our review highlights the importance of addressing infrastructural challenges, ensuring usability of testing devices, and adequately supporting staff through training and workload management to realize the full potential of this new opportunity. Future implementation should consider these factors to successfully integrate molecular diagnostics into community-level healthcare delivery, particularly in rural and remote areas.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.020
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMetaresearch
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Systematic review · Consensus signal: Systematic review
GenreCandidate signal: Review · Consensus signal: Review
Teacher disagreement score0.020
Threshold uncertainty score0.988

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.020
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0020.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.108
GPT teacher head0.432
Teacher spread0.324 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designSystematic review
Domainnot available
GenreReview

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations2
Published2025
Admission routes2
Has abstractyes

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