Genomics and Transcriptomics of 3ANX (NX-2) and NX (NX-3) Producing Isolates of Fusarium graminearum
Bibliographic record
Abstract
Fusarium head blight (FHB) is an important fungal disease caused by Fusarium graminearum and other Fusarium spp., resulting in significant yield losses across cereal grains. Recently identified F. graminearum isolates in Canada, capable of producing type A trichothecene mycotoxins 3ANX (NX-2, 7-α hydroxy,15-deacetylcalonectrin) and NX (NX-3, 7-α hydroxy, 3,15-dideacetylcalonectrin), demonstrated high levels of genetic diversity. While recent studies have detected this genetic and chemical diversity, little is known of the underlying molecular mechanisms and processes influenced by these distinct chemotypes and regional populations. In the current study, we used an -omics approach coupled with high-resolution mass spectrometry to characterize twenty F. graminearum isolates collected from five distinct regions across Manitoba. These data identified regional F. graminearum populations within Manitoba that demonstrate distinct genomic variation and patterns of gene expression, particularly within pathogenicity-associated processes. Further, we identified genetic variation and differential expression between isolates showing high and low levels of pathogenicity, allowing for the identification of previously characterized and novel putative pathogenicity factors. Lastly, we detected the production of 3ANX and/or NX mycotoxins within the majority of our twenty characterized F. graminearum isolates, suggesting the 3ANX chemotype may be more prevalent than previously expected in Canada. Ultimately, these findings highlight the diversity of F. graminearum across Manitoba and, more importantly, uncover specific genomic regions and candidate pathogenicity factors influenced by this diversity.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".