Genome-wide long non-coding RNA expression profile and its regulatory role in the ileocecal valve from Mycobacterium avium subsp. paratuberculosis-infected cattle
Bibliographic record
Abstract
Bovine paratuberculosis (PTB) is a chronic enteritis caused by Mycobacterium avium subsp. paratuberculosis (MAP), which results in significant economic losses to the dairy industry worldwide. Long non-coding RNAs (lncRNAs) play a crucial role in regulating the host immune response due to their interaction with transcripts in proximity. However, their annotation in cattle remains limited, and their role in cattle naturally infected with MAP has not been fully explored. In this study, lncRNAs were identified in the transcriptome of ileocecal valve samples from control cows without lesions ( N = 4) and with PTB-associated focal ( N = 5) and diffuse ( N = 5) lesions in intestinal tissues using RNA sequencing. The raw reads were uploaded into the CLC Bio Genomics Workbench, and the trimmed reads were mapped to the Bos taurus ARS_UCD1.2.109 reference genome using the Large Gap Read Mapping tool. The resulting annotation allowed the identification of 1,434 LncRNAs, 899 of which were novel, using the FlExible Extraction of LncRNA pipeline. LncRNA differential expression (DE) analysis performed with DESeq2 allowed the identification of 1, 6, and 2 DE lncRNAs in the comparisons of cows with focal lesions versus ( vs ) controls, diffuse lesions vs. controls, and diffuse vs. focal lesions, respectively. Best lncRNA partner analysis identified expression correlations between the lncRNA1086.1 , lncRNA ENSBTAG00000050406 , and lncRNA_2340.1 , and the Inactive Phosphatidylinositol 3-Phosphatase 9 ( MTMR9 ), GM Domain Family member B (RGMB), and the homeobox A6 ( HOXA6 ), respectively. The MTMR9 negatively regulates apoptosis, the RGMB positively regulates IL-6 expression, and the HOXA6 regulates cell differentiation and inflammation. The results of the quantitative trait locus (QTL) enrichment analysis showed that the DE lncRNAs were located in genomic regions previously associated with clinical mastitis, HDL cholesterol, bovine tuberculosis, paratuberculosis, and bovine leukosis susceptibility. The identified DE lncRNAs could allow the development of novel PTB diagnostic tools and have potential applications in breeding strategies for PTB-resistant cattle.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.002 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".