Distribution of polyamines in kernel tissues of Canadian barley cultivars and their association with protein content
Why this work is in the frame
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Bibliographic record
Abstract
Polyamines are nitrogen-containing compounds that naturally occur in barley grain and are known to have beneficial effects on human health. This study investigated the distribution of polyamines in barley grains from two covered and two naked Canadian barley cultivars. Five pearling fractions were collected at 5% intervals of kernel weight for all cultivars. Grains from covered cultivars were dehulled before pearling, followed by consecutive sieving to recover the germ, fine hull (<0.5 mm), and coarse hull (>0.5 mm) fractions. The levels of putrescine, spermidine, and spermine in barley kernels with 25% kernel weight removed were 69–76, 55–64, and 21–41% lower than those in unpearled kernels. Dehulled and pearled kernels were enriched in spermine and depleted in putrescine. The barley germ showed a notably high total polyamine content (52.0–65.5 mg/100 g). The protein and total polyamine content in the fine hull fraction were 2.8–3.5 and 2.1–3.3 fold higher, respectively, compared to the coarse hull fraction, reflecting differences in their histological composition as shown by microscopic examination. Correlation analysis revealed positive and significant correlations between polyamine and protein content at the sub-kernel level ( r = 0.769–0.839), suggesting a potential chemical and physiological synchronization between these components during barley seed development and germination. The insights provided by this study on the obtainment of polyamine-rich fractions from barley grains could be valorized for nutraceutical purpose. The understanding about polyamine–protein relationship in barley tissues could aid the selection of cultivating or processing conditions for different uses.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it