Unstable anthocyanin pigmentation in <i>Streptocarpus</i> sect. <i>Saintpaulia</i> (African violet) is due to transcriptional selectivity of a single <scp>MYB</scp> gene
Bibliographic record
Abstract
Saintpaulia (African violet) pigmentation is notoriously unstable and sometimes forms white stripes, particularly following passage through tissue culture. White-striped petals were thought to be due to periclinal chimeras, but we confirmed that white stripes result from epigenetic regulation rather than periclinal chimeras based on the flower color traits of plants obtained from tissue culture. Gene expression in several plant lines, anthocyanin quantification, bisulfite sequencing, and methylation analyses were used to demonstrate the presence of a single MYB gene responsible for pigment variation. We identified SiMYB2 as the cause of variations in tissue color patterning, and that two RNAs were generated from SiMYB2. SiMYB2-Long was expressed in colored tissues, while SiMYB2-Short was expressed only in noncolored tissues. Functional analyses revealed that SiMYB2-Long is an anthocyanin biosynthesis activator and SiMYB2-Short is nonfunctional. Exon 3 of SiMYB2 was generated by the insertion of a transposon-like sequence. A mutant lacking the element was obtained from cultivars with noncolored tissues. Anthocyanin content and SiMYB2-Long expression in the mutant were greatly increased compared to wild-type. Our results suggest that the white-striped petals of Saintpaulia are not formed by periclinal chimeras but through the transcriptional selectivity of epigenetically regulated SiMYB2.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".