A second gene for resistance to four pathotypes of <i>Plasmodiophora brassicae</i> identified in the <i>Brassica napus</i> cultivar ‘Mendel’
Bibliographic record
Abstract
Clubroot, caused by the obligate parasite Plasmodiophora brassicae, poses a significant threat to the Canadian canola industry, resulting in substantial yield losses. Genetic resistance is an effective strategy for managing the disease. In this study, 137 doubled haploid (DH) lines derived from the European oilseed rape cultivar ‘Mendel’ were phenotyped against P. brassicae pathotypes 3D, 5C, and 8J, which are aggressive toward the first-generation clubroot-resistant canola cultivars, and were genotyped using genotyping-by-sequencing. Disease severity indices in the population were highly correlated among the three pathotypes, with correlation coefficients of r ≥ 0.82. In the DH population, 2642 high-quality Single nucleotide polymorphisms were detected by employing the Brassica napus ‘ZS11’ reference genome. A single quantitative trait locus, Rcr3Mendel, was detected and mapped to a 317 kb region on chromosome A08, flanked by the markers ZS_A08_15999175 and ZS_A08_16316110. Within this region, 43 genes were identified, including a single Toll interleukin-1 receptor nucleotide-binding site-leucine-rich repeat (TNL) gene, BnaA08T0102200ZS. This region is homologous to the 13 415 472–15 791 728 bp region of chromosome A08 in Brassica rapa ECD 04, the donor of clubroot resistance in ‘Mendel’. Two TNL genes, BraA08g039211E and BraA08g039212E, were identified in the ECD 04 genome. Kompetitive allele-specific PCR analysis identified eight markers that co-segregated with Rcr3Mendel. Resistance to pathotype 3H was also found to co-segregate with resistance to pathotypes 5C, 3D, and 8J.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".