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Record W4411522469 · doi:10.1016/j.ympev.2025.108397

Genomic insights into wheatgrass: unravelling genetic diversity, population structure, and evolutionary dynamics in Pseudoroegneria species

2025· article· en· W4411522469 on OpenAlexafffund
Nadeem Khan, Raju Chaudhary, Sampath Perumal, Zhengping Wang, Pierre Hucl, Bill Biligetu, Andrew Sharpe, Lingling Jin

Bibliographic record

VenueMolecular Phylogenetics and Evolution · 2025
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicPlant Taxonomy and Phylogenetics
Canadian institutionsUniversity of SaskatchewanGlobal Institute for Water Security
FundersSaskatchewan Cattlemen's AssociationNatural Sciences and Engineering Research Council of CanadaAgricultural Development FundMinistry of Agriculture - Saskatchewan
KeywordsBiologyGenetic diversityOutcrossingEvolutionary biologyPopulationGenetic structureGene flowGenetic variationNucleotide diversityPhylogenetic treeEcologyGeneticsAlleleGeneHaplotype

Abstract

fetched live from OpenAlex

Pseudoroegneria species are outcrossing perennial grasses with significant ecological and agricultural value due to its resilience in challenging environmental conditions in the temperate regions. Understanding the extent and pattern of genetic diversity and population structure is essential for the breeding improvement and conservation of these species. In the current research, we analyzed genomic variations of 145 genotypes representing seven species (P. spicata, P. tauri, P. geniculata, P. libanotica, P. strigosa, P. stipifolia, and P. cognata) from major global lineages, using genotyping-by-sequencing. Different metrics including nucleotide diversity, observed heterozygosity, and Tajima's D were used to evaluate genetic diversity among populations revealing significant variation among species, with P. stipifolia exhibiting the highest diversity and P. spicata the lowest. Population structure analyses, including principal component analysis, admixture inference, and phylogenetic reconstruction, identified six distinct genetic clusters, with P. spicata (a North American species) clearly separated from the other six species underscoring its unique genetic identity. In contrast, the Eurasian species exhibit mixed ancestry, indicating intricate genetic relationships and widespread exchange of genetic material. Furthermore, no single species tree fully captures the relationships among them, implying interactions such as hybridization or gene flows between closely related species. To investigate the evolutionary history of Eurasian Pseudoroegneria species, we reconstructed the species tree topology based on the SNV (single nucleotide variants) matrix, and f-branch statistics confirmed close relationships among P. spicata, P. libanotica, and P. tauri, with evidence of unidirectional gene flow between P. stipifolia, P. geniculata, and P. strigosa. These findings highlight the importance of high genetic variation in the P. stipifolia population, suggesting its potential to be a reservoir of valuable alleles for resilient genotypes.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.199
Threshold uncertainty score0.442

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.007
GPT teacher head0.176
Teacher spread0.169 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes2
Has abstractyes

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