Role of the clock gene homolog aha-1 in the circadian system of Caenorhabditis elegans
Bibliographic record
Abstract
Background Circadian rhythms are endogenous and allow organisms to adapt to external daily rhythms of light, temperature and other environmental factors. Circadian rhythms are regulated by a central clock, which is based on a transcription-translation feedback loop. The AHA-1 protein from Caenorhabditis elegans possesses all conserved domains and shows high homology with the positive elements of the central clock loop, BMAL1 in mammals and CYCLE in Drosophila. Methods We studied the possible involvement of aha-1 in the circadian system of adult C. elegans using a bioluminescence-based circadian transcriptional reporter. We also performed qPCRs experiments to quantify the mRNA levels for the aha-1 gene from bulk RNA extractions from adult worms. Results We observed robust luminescent circadian rhythms driven by the aha-1 promoter. However, aha-1 mRNA levels did not show circadian oscillation under the conditions tested. We also show that a mutation in aha-1 generates a significantly longer endogenous period than the one in control strains, suggesting a role for this gene in the nematode circadian clock. Conclusion The results indicate that the CYCLE/BMAL-1 homolog AHA-1 plays a key role in the generation of circadian rhythms in adult nematodes, mainly by regulating period length. These results suggest that the molecular control of circadian regulation in C. elegans exhibits some similarities to other clock model systems.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".