Bibliographic record
Abstract
The aim of this study was to estimate the effect of concentration, volume and temperature of koi carp (Cyprinus carpio koi) serum complement on unsensitized rabbit red blood cell hemolysis.The complement system is known to be a critical component of innate immunity.Serum complement is valuable tool in determining the health status of fish.The alternative pathway activity of complement system can be measured by serum hemolytic activity activated in foreign red blood cells.The zones of hemolysis were clearly visible and were measured manually.The results indicated that Serum concentrations of 12.5% v/v produced 3.9±0.05mm,25%, 4.1±0.115mm,50%, 4.9±0.05mm and 100%, 6.8±0.4 mm hemolysis.10μL volume of serum resulted in 5.25±0.28mm,20μl, 6.1 ±0.23 mm and 30μL 6.7±0.25mmhemolysis.Incubation of sera at 4°C produced 6±0.08mm, 25°C, 6.25±0.19mmbut at 37°C 5.8±0.25mmhemolysis.From the result of the present study, we concluded that the alternative complement pathway activity in Cyprinus carpio koi serum could be affected by concentration, volume and temperature.This is the first report on the innate immune activity of Cyprinus carpio koi, and provides useful data for future assessments of fish health.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.002 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.002 | 0.001 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.003 | 0.001 |
| Open science | 0.002 | 0.002 |
| Research integrity | 0.004 | 0.002 |
| Insufficient payload (model declined to judge) | 0.839 | 0.851 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; the direct Gemma label and the distilled Codex classifier agree on what is shown here.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".