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Record W4411722783 · doi:10.1139/cjm-2025-0061

RNA-seq analysis of transcription patterns during infection of <i>Mesorhizobium japonicum</i> by phage Cp1R7A-A1

2025· article· en· W4411722783 on OpenAlexafffundvenue
K. M. Damitha Gunathilake, Kara Dawn Loos, Christopher K. Yost, Michael F. Hynes

Bibliographic record

VenueCanadian Journal of Microbiology · 2025
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicLegume Nitrogen Fixing Symbiosis
Canadian institutionsUniversity of ReginaUniversity of Calgary
FundersNatural Sciences and Engineering Research Council of Canada
KeywordsBiologyGeneTranscription (linguistics)GeneticsRNAGene expressionTransfer RNAMolecular biology

Abstract

fetched live from OpenAlex

RNA-Seq analysis of Mesorhizobium japonicum infected by phage Cp1R7A-A1 (full name vB_MloS_Cp1R7A-A1) revealed the transcription of all 237 putative phage genes, including 27 tRNA and 210 protein-encoding genes. All predicted protein-encoding genes of Cp1R7A_A1 showed expression. The phage tRNA genes also showed a notable expression, indicating a possible tRNA-dependant phage survival strategy. Host genes related to nutrient uptake and energy metabolism were significantly upregulated within 1–2 h post infection, likely reflecting the phage's metabolic demands. Notably, peptidoglycan-cleaving enzymes showed elevated expression after 3 h, suggesting host stress responses. These findings show the transcriptional interplay between phage Cp1R7A-A1 and M. japonicum, providing a foundation for future functional studies.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.004
Threshold uncertainty score0.007

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.006
GPT teacher head0.191
Teacher spread0.185 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes3
Has abstractyes

Explore more

Same venueCanadian Journal of Microbiology→Same topicLegume Nitrogen Fixing Symbiosis→French-language works237,207→