Renal phosphorylation of p44 mitogen-activated protein kinase and myosin light chain phosphatase targeting subunit 1 at Thr697 as cadmium-induced hypertension mechanisms
Bibliographic record
Abstract
Objective: Cadmium (Cd) exposure is implicated in the pathogenesis of hypertension, a global non-communicable lifestyle disease which is reportedly mitigated by potassium supplementation. We aimed to induce hypertension in male Sprague-Dawley rats to investigate the mechanisms of Cd-induced hypertension and the mitigating effects of potassium supplementation since they are not fully elucidated. Hypothesis: We hypothesized that Cd induces hypertension through activation of extracellular signal-regulated kinase (ERK) pathways and inhibition of myosin light chain phosphatase (MLCP). METHOD: Six cohorts of control, Cd-exposed, and potassium-supplemented male Sprague-Dawley rats were selected. Hypertension was induced using cadmium chloride (2.5 or 5 mg/kg b.w.). Blood pressure, heart rate, and blood flow were measured twice weekly. Protein expressions were assessed in Cd-exposed rats for eight weeks via western blotting to determine the calcium handling effect and possible signaling pathways and underlying mechanisms involved. Data: Statistical analysis was done using one-way analysis of variance (ANOVA) and the results were reported as mean ± standard error of the mean. The Games-Howell or Bonferroni post hoc test was used for multiple comparisons. Summary of Results: Cd induced hypertension in vivo by significantly (p < 0.001) elevating blood pressures (mm Hg) [systolic (160 ± 2 and 155 ± 1 vs 120 ± 1), diastolic (119 ± 2 and 110 ± 1 vs 81 ± 1), mean arterial (133 ± 2 and 125 ± 1 vs 94 ± 1), and pulse (43 ± 1 and 45 ± 1 vs 39 ± 1; p < 0.05)], heart rate (507.2 ± 6.7 Cd 5 mg/kg b.w. vs 467.5 ± 7.5 beats/minute; p < 0.05), and flow rate (26.25 ± 1.20 and 28.28 ± 1.24 vs 18.02 ± 0.74 mL/L) while potassium supplementation conferred protection. The mechanism involved augmenting renal myosin light chain phosphatase targeting subunit 1 at threonine 697 (MYPT1-Thr697) (2.58 ± 0.36 vs 1 ± 0) and p44 mitogen-activated protein kinase (MAPK) (1.78 ± 0.20 vs 1 ± 0). Potassium supplementation significantly reduced this MYPT1-Thr697 phosphorylation. Conclusions: The results show that the hypertensive effects of Cd could be mediated by MLCP inhibition via phosphorylation of renal MYPT1-Thr697 and oxidative stress via p44 MAPK, an ERK. The importance of the kidneys in regulating blood pressure changes after Cd exposure and the mitigating effects of potassium supplementation are also emphasized. The kidneys should, therefore, be targeted for drug therapy in managing hypertension and further investigation of small arteries and Rho-kinase/MYPT1 interactions is recommended. Additionally, the study was limited by not challenging the tissues with a contractile agent for LC20 analysis and not being able to perform blots for Rho-kinase, so future studies should also include these two assessments. The Mona Campus Committee for Research and Publications and Graduate Awards, School for Graduate Studies and Research, The University of the West Indies This abstract was presented at the American Physiology Summit 2025 and is only available in HTML format. There is no downloadable file or PDF version. The Physiology editorial board was not involved in the peer review process.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".