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SCALING COWPOX VACCINE PRODUCTION TECHNOLOGY: FROM DEVELOPMENT TO GMP STANDARDS

2025· article· en· W4412008110 on OpenAlexaff
Kuandyk Zhugunissov, Kuznetsova S.N., Kharytonov Mykola M., B. M. Ismagambetov, Yergali Abduraimov, Aralbek Rsaliyev, Nurgul Sikhayeva, Zakir Yershebulov

Bibliographic record

VenueĠylym ža̋ne bìlìm · 2025
Typearticle
Languageen
FieldImmunology and Microbiology
TopicPoxvirus research and outbreaks
Canadian institutionsDebiopharm Group (Canada)
Fundersnot available
KeywordsCowpoxProduction (economics)VirologyComputer scienceBiologyGeneticsRecombinant DNAVacciniaEconomics

Abstract

fetched live from OpenAlex

This paper presents a comprehensive analysis and systematization of the key stages of scaling the technological chain of production of cowpox vaccine, with an emphasis on the full-scale adaptation of production processes to strict criteria of good manufacturing practice (GMP). Special attention is paid to the detailed validation of all key stages, including virus cultivation, vaccine formation, lyophilization process and comprehensive quality control of the final product. Validation of key processes was carried out with careful elaboration of virus culture protocols, which included the use of the Nunc Cell Factory, ensuring high stability and reproducibility of the procedure. The introduction of improved methods of microbiological purity control at all stages of production, including regular checks for the presence of microorganisms and monitoring of environmental parameters, contributed to improving the reliability of the production process. Operational standardization was achieved through the integration of real-time systems for data monitoring and analysis, which helped minimize human error and improve production accuracy. As a result, key optimization strategies have been identified and implemented to improve production efficiency, including improving automation processes and digitalizing production lines. The results of the study represent a significant step forward in the development of veterinary vaccinology, providing a reliable basis for future developments and innovations in the field of prevention and treatment of infectious diseases in farm animals. The work highlights the importance of an interdisciplinary approach combining biological, engineering, and management competencies to achieve high standards of vaccine quality and safety. In light of the emerging challenges associated with changes in legislation and the development of new technologies, this work offers a platform for continuing research in the field of biotechnology and vaccine production aimed at adapting to the dynamic conditions of the veterinary industry.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.010
metaresearch head score (Gemma)0.007
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Methods · Consensus signal: Methods
Teacher disagreement score0.010
Threshold uncertainty score0.054

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0100.007
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0020.002
Science and technology studies0.0010.002
Scholarly communication0.0050.004
Open science0.0010.003
Research integrity0.0010.002
Insufficient payload (model declined to judge)0.0010.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.009
GPT teacher head0.287
Teacher spread0.278 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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