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Record W4412487973 · doi:10.1002/jev2.70120

Roadblocks of Urinary EV Biomarkers: Moving Toward the Clinic

2025· review· en· W4412487973 on OpenAlex
Marvin Droste, Maija Puhka, Martin E. van Royen, Monica Suet Ying Ng, Charles J. Blijdorp, Gloria Álvarez‐Llamas, Francesc E. Borràs, Anja Büscher, Benedetta Bussolati, James W. Dear, Juan Manuel Falcón‐Pérez, Bernd Giebel, Cristina Grange, Ewout J. Hoorn, Janne Leivo, Metka Lenassi, Alicia Llorente, Fabrice Lucien, Inge Mertens, Harald Mischak, Desmond Pink, Tobias Tertel, Swasti Tiwari, Dolores Di Vizio, Peter S.T. Yuen, Nataša Zarovni, Guido Jenster, Dylan Burger, Elena S. Martens‐Uzunova, Uta Erdbrügger

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueJournal of Extracellular Vesicles · 2025
Typereview
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicExtracellular vesicles in disease
Canadian institutionsUniversity of OttawaAlberta Oil Sands Technology and Research Authority
FundersNational Cancer InstituteNational Institute of Diabetes and Digestive and Kidney DiseasesInstituto de Salud Carlos IIINational Institutes of HealthJavna Agencija za Raziskovalno Dejavnost RSEuropean Regional Development FundEuropean CommissionKidney Foundation of CanadaCanadian Institutes of Health ResearchAmerican Diabetes AssociationQueensland HealthH2020 European Research CouncilFundación Mutua MadrileñaMetro North Hospital and Health ServiceCanadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of CanadaComunidad de MadridCenter for Cancer Research
KeywordsStatus quoBiomarkerExtracellular vesiclesIdentification (biology)Computer scienceMedicineEngineering ethicsRisk analysis (engineering)Political scienceBiologyEngineering

Abstract

fetched live from OpenAlex

Despite remarkable interest in the biomarker potential of urinary extracellular vesicles (uEVs) and the identification of numerous promising candidates, their clinical translation still presents multiple challenges. The opportunities for successful translation are obvious, yet the main roadblocks on the way have hardly been systematically considered and more coordinated approaches are needed to overcome them. In the present review article, we have identified the most relevant roadblocks of clinical translation of urinary EV-based biomarkers and discuss possible solutions to overcome them. These roadblocks are categorized as fundamental and technical but also related to development of novel biomarker assays and clinical acceptance. In addition, hurdles within the regulatory approval process are discussed. It is clear that various roadblocks to clinical translation of urinary EV biomarkers exist; however, they are addressable by promoting rigor and reproducibility as well as collaboration between basic and clinical scientists, clinicians, industry and regulatory bodies. Moreover, knowledge of obstacles for assay development and regulatory requirements should already be considered when developing a new biomarker to maximize the chance of successful translation. This review presents not only a status quo, but also a roadmap for the further development of the field.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.003
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Review · Consensus signal: Review
Teacher disagreement score0.965
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0030.001
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0020.002
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0020.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.033
GPT teacher head0.329
Teacher spread0.297 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it